Loading…
Rapid nested-PCR for tyrosinase gene detection on chip
The availability of non-invasive, fast and sensitive technologies for detection of circulating cancer cells is still a critical need of clinical oncology, particularly for diagnosis of aggressive and highly metastatic tumors, like malignant melanoma. Here we present the first nested polymerase chain...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Default Article |
| Published: |
2011
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/2134/17817 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1818172870884327424 |
|---|---|
| author | Anna G. Sciancalepore Alessandro Polini Elisa Mele Salvatore Girardo Roberto Cingolani Dario Pisignano |
| author_facet | Anna G. Sciancalepore Alessandro Polini Elisa Mele Salvatore Girardo Roberto Cingolani Dario Pisignano |
| author_sort | Anna G. Sciancalepore (7125557) |
| collection | Figshare |
| description | The availability of non-invasive, fast and sensitive technologies for detection of circulating cancer cells is still a critical need of clinical oncology, particularly for diagnosis of aggressive and highly metastatic tumors, like malignant melanoma. Here we present the first nested polymerase chain reaction process carried out by a microfabricated, hybrid plastic–glass microfluidic chip on the tyrosinase gene, a predictive marker for melanoma diagnosis. The device is a hybrid system consisting of a glass microchannel embedded in an elastomeric matrix, and operating in flow-oscillating modality on a droplet of biological sample. The convection heat transfer and the temperature distribution inside the carrier fluid in the device are investigated. The oil responds to temperature changes with a characteristic time around 53 s, and exhibits three different thermal gradients along the capillary, with temperature variations below 4 ◦C in correspondence of heater electrodes. The sample heating/cooling rates in the chip are as high as 16 ◦C/s, allowing rapid processes. The nested polymerase chain reaction process is performed in less than 50 min, namely more than four times faster than in a standard thermocycler. The rapidity of the analysis method, combined with the simple and low-cost fabrication, reduced sample evaporation, and flexibility of the overall microfluidic platform, make it promising for the detection of events of tumor spreading. |
| format | Default Article |
| id | rr-article-9236897 |
| institution | Loughborough University |
| publishDate | 2011 |
| record_format | Figshare |
| spelling | rr-article-92368972011-01-01T00:00:00Z Rapid nested-PCR for tyrosinase gene detection on chip Anna G. Sciancalepore (7125557) Alessandro Polini (201388) Elisa Mele (1251579) Salvatore Girardo (517120) Roberto Cingolani (189795) Dario Pisignano (201394) Materials engineering not elsewhere classified Tyrosinase Microfluidics Nested-PCR Melanoma Materials Engineering not elsewhere classified The availability of non-invasive, fast and sensitive technologies for detection of circulating cancer cells is still a critical need of clinical oncology, particularly for diagnosis of aggressive and highly metastatic tumors, like malignant melanoma. Here we present the first nested polymerase chain reaction process carried out by a microfabricated, hybrid plastic–glass microfluidic chip on the tyrosinase gene, a predictive marker for melanoma diagnosis. The device is a hybrid system consisting of a glass microchannel embedded in an elastomeric matrix, and operating in flow-oscillating modality on a droplet of biological sample. The convection heat transfer and the temperature distribution inside the carrier fluid in the device are investigated. The oil responds to temperature changes with a characteristic time around 53 s, and exhibits three different thermal gradients along the capillary, with temperature variations below 4 ◦C in correspondence of heater electrodes. The sample heating/cooling rates in the chip are as high as 16 ◦C/s, allowing rapid processes. The nested polymerase chain reaction process is performed in less than 50 min, namely more than four times faster than in a standard thermocycler. The rapidity of the analysis method, combined with the simple and low-cost fabrication, reduced sample evaporation, and flexibility of the overall microfluidic platform, make it promising for the detection of events of tumor spreading. 2011-01-01T00:00:00Z Text Journal contribution 2134/17817 https://figshare.com/articles/journal_contribution/Rapid_nested-PCR_for_tyrosinase_gene_detection_on_chip/9236897 CC BY-NC-ND 4.0 |
| spellingShingle | Materials engineering not elsewhere classified Tyrosinase Microfluidics Nested-PCR Melanoma Materials Engineering not elsewhere classified Anna G. Sciancalepore Alessandro Polini Elisa Mele Salvatore Girardo Roberto Cingolani Dario Pisignano Rapid nested-PCR for tyrosinase gene detection on chip |
| title | Rapid nested-PCR for tyrosinase gene detection on chip |
| title_full | Rapid nested-PCR for tyrosinase gene detection on chip |
| title_fullStr | Rapid nested-PCR for tyrosinase gene detection on chip |
| title_full_unstemmed | Rapid nested-PCR for tyrosinase gene detection on chip |
| title_short | Rapid nested-PCR for tyrosinase gene detection on chip |
| title_sort | rapid nested-pcr for tyrosinase gene detection on chip |
| topic | Materials engineering not elsewhere classified Tyrosinase Microfluidics Nested-PCR Melanoma Materials Engineering not elsewhere classified |
| url | https://hdl.handle.net/2134/17817 |