On the application of 'seeding' techniques in the primary separation of plasmid DNA from neutralised E-coli lysates

BACKGROUND: Initial extraction of plasmid DNA from Escherichia coli and its separation from host-derived contaminants is a difficult task to perform. Here, we examine the application of particle ‘seeding’ solid-liquid separation methods for primary recovery of plasmid DNA from neutralised alkaline c...

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Bibliographic Details
Main Authors: Eirini Theodosiou, Owen R.T. Thomas
Format: Default Article
Published: 2008
Subjects:
Chemical engineering not elsewhere classified
Alkaline lysis
Fillers
Flotation
Gene therapy
Genetic vaccination
Magnetic particle seeds
Low-density gas-filled microspheres
Chemical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/12016
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