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Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study
© 2018 Elsevier B.V. The main goal of this study was to produce a novel porous scaffold for rapid in vivo bone healing behavior. Lyophilization technique was used to produce this highly porous hybrid scaffold from Na-alginate (S) and hydroxyethylcellulose (HEC) impregnated with different concentrati...
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| Main Authors: | , , , , , , |
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| Format: | Default Article |
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2018
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| Online Access: | https://hdl.handle.net/2134/36247 |
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| _version_ | 1818169032010891264 |
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| author | Khairy M. Tohamy Islam E. Soliman Mostafa Mabrouk Shaimaa ElShebiney Hanan H. Beherei Mohamed A. Aboelnasr Diganta Das |
| author_facet | Khairy M. Tohamy Islam E. Soliman Mostafa Mabrouk Shaimaa ElShebiney Hanan H. Beherei Mohamed A. Aboelnasr Diganta Das |
| author_sort | Khairy M. Tohamy (7130126) |
| collection | Figshare |
| description | © 2018 Elsevier B.V. The main goal of this study was to produce a novel porous scaffold for rapid in vivo bone healing behavior. Lyophilization technique was used to produce this highly porous hybrid scaffold from Na-alginate (S) and hydroxyethylcellulose (HEC) impregnated with different concentration of hydroxyapatite (HA). After cross-linking the scaffolds, their incubation was carried out in simulated body fluid (SBF) for 4 weeks at 37 °C to investigate their bioactivity. A number of techniques were employed (e.g., XRD, FTIR, SEM, EDX, and texture analyzer) to characterize the designed scaffolds. It was observed that the mechanical properties of the scaffolds increase deformation energy (182 ± 16 J/m3) and rigidity gradient (19.44 ± 0.85 Pa) after loading with HA. Furthermore, the scaffolds were implanted in femur critical size defects (2 mm) of adult male Wistar rats for 6 weeks. In vitro and in vivo analyses demonstrated impressive bioactivity and biocompatibility for the prepared scaffolds, especially those containing HA. Based on the obtained results we conclude that the designed scaffolds are promising solutions for bone regeneration applications. |
| format | Default Article |
| id | rr-article-9244718 |
| institution | Loughborough University |
| publishDate | 2018 |
| record_format | Figshare |
| spelling | rr-article-92447182018-07-21T00:00:00Z Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study Khairy M. Tohamy (7130126) Islam E. Soliman (7130129) Mostafa Mabrouk (7129652) Shaimaa ElShebiney (7130132) Hanan H. Beherei (7129646) Mohamed A. Aboelnasr (7130135) Diganta Das (1254000) Chemical engineering not elsewhere classified Porous hybrid scaffolds Polysaccharides Biocompatibility Mechanical properties Rapid bone regeneration Chemical Engineering not elsewhere classified © 2018 Elsevier B.V. The main goal of this study was to produce a novel porous scaffold for rapid in vivo bone healing behavior. Lyophilization technique was used to produce this highly porous hybrid scaffold from Na-alginate (S) and hydroxyethylcellulose (HEC) impregnated with different concentration of hydroxyapatite (HA). After cross-linking the scaffolds, their incubation was carried out in simulated body fluid (SBF) for 4 weeks at 37 °C to investigate their bioactivity. A number of techniques were employed (e.g., XRD, FTIR, SEM, EDX, and texture analyzer) to characterize the designed scaffolds. It was observed that the mechanical properties of the scaffolds increase deformation energy (182 ± 16 J/m3) and rigidity gradient (19.44 ± 0.85 Pa) after loading with HA. Furthermore, the scaffolds were implanted in femur critical size defects (2 mm) of adult male Wistar rats for 6 weeks. In vitro and in vivo analyses demonstrated impressive bioactivity and biocompatibility for the prepared scaffolds, especially those containing HA. Based on the obtained results we conclude that the designed scaffolds are promising solutions for bone regeneration applications. 2018-07-21T00:00:00Z Text Journal contribution 2134/36247 https://figshare.com/articles/journal_contribution/Novel_polysaccharide_hybrid_scaffold_loaded_with_hydroxyapatite_Fabrication_bioactivity_and_in_vivo_study/9244718 CC BY-NC-ND 4.0 |
| spellingShingle | Chemical engineering not elsewhere classified Porous hybrid scaffolds Polysaccharides Biocompatibility Mechanical properties Rapid bone regeneration Chemical Engineering not elsewhere classified Khairy M. Tohamy Islam E. Soliman Mostafa Mabrouk Shaimaa ElShebiney Hanan H. Beherei Mohamed A. Aboelnasr Diganta Das Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study |
| title | Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study |
| title_full | Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study |
| title_fullStr | Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study |
| title_full_unstemmed | Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study |
| title_short | Novel polysaccharide hybrid scaffold loaded with hydroxyapatite: Fabrication, bioactivity, and in vivo study |
| title_sort | novel polysaccharide hybrid scaffold loaded with hydroxyapatite: fabrication, bioactivity, and in vivo study |
| topic | Chemical engineering not elsewhere classified Porous hybrid scaffolds Polysaccharides Biocompatibility Mechanical properties Rapid bone regeneration Chemical Engineering not elsewhere classified |
| url | https://hdl.handle.net/2134/36247 |