Loading…
Biochemical and pharmacological studies of the metabolism of dynorphin 1-8
The metabolism of the opioid peptide [3H]dynorphin 1-8 by slices of central nervous system (c.n.s.) and peripheral tissues, from the rat and guinea-pig has been studied. Rat spinal cord rapidly degraded [3H]dynorphin 1-8, the N-terminal tyrosine residue being most susceptible to hydrolysis and there...
Saved in:
| Main Author: | |
|---|---|
| Format: | Default Thesis |
| Published: |
1990
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/2134/32333 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1818169807534555136 |
|---|---|
| author | Diane M. Dixon |
| author_facet | Diane M. Dixon |
| author_sort | Diane M. Dixon (7167092) |
| collection | Figshare |
| description | The metabolism of the opioid peptide [3H]dynorphin 1-8 by slices of central nervous system (c.n.s.) and peripheral tissues, from the rat and guinea-pig has been studied. Rat spinal cord rapidly degraded [3H]dynorphin 1-8, the N-terminal tyrosine residue being most susceptible to hydrolysis and therefore forming the major metabolite. Pre-treatment of the metabolizing tissue with a standard cocktail of enzyme inhibitors decreased the degradation of [3H]dynorphin 1-8 at both the N- and C-termini. However, inclusion of this enzyme inhibitor cocktail revealed the activity of a further enzyme, an endopeptidase, capable of cleaving the leucine5–arginine6 bond within the octapeptide liberating the opioid pentapeptide [Leu]enkephalin. This pattern of metabolism was observed across all rat brain regions and periphery. [Continues.] |
| format | Default Thesis |
| id | rr-article-9399302 |
| institution | Loughborough University |
| publishDate | 1990 |
| record_format | Figshare |
| spelling | rr-article-93993021990-01-01T00:00:00Z Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 Diane M. Dixon (7167092) Other chemical sciences not elsewhere classified untagged Chemical Sciences not elsewhere classified The metabolism of the opioid peptide [3H]dynorphin 1-8 by slices of central nervous system (c.n.s.) and peripheral tissues, from the rat and guinea-pig has been studied. Rat spinal cord rapidly degraded [3H]dynorphin 1-8, the N-terminal tyrosine residue being most susceptible to hydrolysis and therefore forming the major metabolite. Pre-treatment of the metabolizing tissue with a standard cocktail of enzyme inhibitors decreased the degradation of [3H]dynorphin 1-8 at both the N- and C-termini. However, inclusion of this enzyme inhibitor cocktail revealed the activity of a further enzyme, an endopeptidase, capable of cleaving the leucine5–arginine6 bond within the octapeptide liberating the opioid pentapeptide [Leu]enkephalin. This pattern of metabolism was observed across all rat brain regions and periphery. [Continues.] 1990-01-01T00:00:00Z Text Thesis 2134/32333 https://figshare.com/articles/thesis/Biochemical_and_pharmacological_studies_of_the_metabolism_of_dynorphin_1-8/9399302 CC BY-NC-ND 4.0 |
| spellingShingle | Other chemical sciences not elsewhere classified untagged Chemical Sciences not elsewhere classified Diane M. Dixon Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| title | Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| title_full | Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| title_fullStr | Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| title_full_unstemmed | Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| title_short | Biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| title_sort | biochemical and pharmacological studies of the metabolism of dynorphin 1-8 |
| topic | Other chemical sciences not elsewhere classified untagged Chemical Sciences not elsewhere classified |
| url | https://hdl.handle.net/2134/32333 |