Generation of human induced pluripotent stem cells using non-synthetic mRNA.

Here we describe some of the crucial steps to generate induced pluripotent stem cells (iPSCs) using mRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribed mRNA. V. virus' 2'-O-Methyltrans...

Full description

Bibliographic Details

Main Authors:	Leili Rohani, Claire Fabian, H. Holland, Yahaira Naaldijk, R. Dressel, H. Loffler-Wirth, Hans Binder, Antje Arnold, Alexandra Stolzing
Format:	Default Article
Published:	2016
Subjects:	Mechanical engineering not elsewhere classified Induced pluripotent stem cell Reprogramming mRNA Mechanical Engineering not elsewhere classified
Online Access:	https://hdl.handle.net/2134/20950
Tags:	Add Tag No Tags, Be the first to tag this record!