Culture of human mesenchymal stem cells on microcarriers in a 5 l stirred-tank bioreactor by Qasim Rafiq, Kathryn Brosnan, Karen Coopman, Alvin W. Nienow, Christopher Hewitt

“…Over 12 days, the dissolved O2 concentration was >45 % of saturation and the pH between 7.2 and 6.7 giving a maximum cell density in the 5 l bioreactor of 1.7 × 105 cells/ml; this represents >sixfold expansion of the hMSCs, equivalent to that achievable from 65 fully-confluent T-175 flasks. …”
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A potentially scalable method for the harvesting of hMSCs from microcarriers by Alvin W. Nienow, Qasim Rafiq, Karen Coopman, Christopher Hewitt

“…Detachment was then successfully achieved for hMSCs from two different donors using microcarrier/cell suspensions up to 100 mL in a spinner flask. …”
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Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms by Alvin W. Nienow, Christopher J. Hewitt, Thomas Heathman, Veronica A.M. Glyn, G. Fonte, Petra Hanga, Karen Coopman, Qasim A. Rafiq

“…In our recent work in different bioreactors up to 2.5 L in scale, we have successfully cultured hMSCs using the minimum agitator speed required for complete microcarrier suspension, NJS. …”
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Supplementary figures for "Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor" by Qasim A. Rafiq, Mariana P. Hanga, Thomas Heathman, Karen Coopman, Alvin Nienow, David Williams, Christopher Hewitt

“…At both scales, the agitation used during culture was the minimum required for microcarrier suspension, NJS. The use of the ambr15, with its improved control compared to the spinner flask, reduced the coefficient of variation on viable cell density in the serum containing medium from 7.65% to 4.08%, and the switch to serum free further reduced these to 1.06–0.54%, respectively. …”
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Development of a process control strategy for the serum-free microcarrier expansion of human mesenchymal stem cells towards cost-effective and commercially viable manufacturing by Thomas R.J. Heathman, Alvin W. Nienow, Qasim A. Rafiq, Karen Coopman, Bo Kara, Christopher J. Hewitt

“…At 25% dO2, there was a 300% increase in the BM-hMSC yield in the bioreactor across the two donor BM-hMSCs in SFM compared to FBS-containing medium. …”
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Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor by Qasim A. Rafiq, Mariana P. Hanga, Thomas R.J. Heathman, Karen Coopman, Alvin W. Nienow, David Williams, Christopher J. Hewitt

“…At both scales, the agitation used during culture was the minimum required for microcarrier suspension, NJS . The use of the ambr15, with its improved control compared to the spinner flask, reduced the coefficient of variation on viable cell density in the serum containing medium from 7.65% to 4.08%, and the switch to serum free further reduced these to 1.06% and 0.54% respectively. …”
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Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum-free microcarrier process by Thomas Heathman, Veronica A.M. Glyn, Andy Picken, Qasim A. Rafiq, Karen Coopman, Alvin W. Nienow, Bo Kara, Christopher J. Hewitt

“…Human bone-marrow derived hMSCs were expanded on fibronectin-coated, non-porous plastic microcarriers in 100 mL stirred spinner flasks at a density of 3 × 105 cells.mL−1 in serum-free medium. …”
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Developing a standardised manufacturing process for the clinical-scale production of human mesenchymal stem cells by Qasim A. Rafiq

“…A maximum cell density of 1.68 x 105 cells/mL was obtained after 9 days in culture; further growth was limited by the low glucose concentration and lack of available surface area. …”
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Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors by Qasim A. Rafiq, Karen Coopman, Alvin W. Nienow, Christopher J. Hewitt

“…Monolayer studies demonstrated input cell line variability with respect to growth kinetics and metabolite flux. HBM-MSC1 underwent more cumulative population doublings over three passages in comparison to hBM-MSC2 and hBM-MSC3. …”
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Developing a process control strategy for the consistent and scalable manufacture of human mesenchymal stem cells by Thomas R.J. Heathman

“…This achieved a maximum cell density of 0.08 ± 0.01 · 106 cells.mL-1 in FBS-based medium, 0.12 ± 0.01 · 106 cells.mL-1 in HPL-based medium and 0.27 ± 0.03· 106 cells.mL-1 in serum free medium after six days. …”
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