Identification and incidence of pepper viruses in north central Chile and its association with vectors

• Published in: Agricultura técnica 2005-10, Vol.65 (3)
• Main Authors: Sepúlveda, Paulina R, Larraín, Patricia S, Quiroz, Carlos E, Rebufel, Patricia A, Graña, Fernando S
• Format: Article
• Language: Spanish
• Subjects: pepper virus, insect vectors, Tomato spotted wilt virus, Cucumber mosaic virus, Alfalfa mosaic virus, Potato virus Y , ELISA; virus en pimiento, insectos vectores, Tomato spotted wilt virus, Cucumber mosaic virus, Alfalfa mosaic virus, Potato virus Y , ELISA
• ISSN: 0365-2807

During the 2001-2002 and 2002-2003 seasons, a virus survey was carried out on peppers ( Capsicum annuum L.) in different locations of the Region of Coquimbo (29,5-32° S lat.). The DAS- ELISA test was used for virus detection. The relative importance of the viruses encountered was as follows: Cucumber mosaic virus (CMV) 23.3%; Tomato spotted wilt virus (TSWV) 20.8%; Alfalfa mosaic virus (AMV) 14.8%; Potato virus Y (PVY) 14.5%; Impatients necrotic spot virus (INSV) 3.1% (first identification in peppers in Chile); Tomato mosaic virus (ToMV) 2.2% and Tobacco mosaic virus (TMV) 4.9% The results also indicated that nearly 30% of the samples were infected with two or more viruses. The incidence of the viruses was different for each location and season, and was affected greatly by the sanitary condition of the plants at transplanting. When healthy plants were used to start a crop, the incidence of viruses at the end of the season was lower. The Western flower thrip, Frankliniella occidentalis (Pergande)was considered as the most important vector of TSWV and INSV, while the Green peach aphid, Myzus persicae (Sulzer), and Potato aphid Macrosiphum eurphobiae (Thomas), were the most important species as vectors of CMV, PVY and AMV. The presence of these viruses in the crops was related directly with the presence of these aphids in traps and on plants. Durante las temporadas de 2001-2002 y 2002-2003 se realizó una prospección de virus en pimientos ( Capsicum annuum L.) en diversas localidades de la Región de Coquimbo (29,5-32° lat. Sur). La determinación de virus se realizó mediante la prueba DAS- ELISA. La importancia relativa de los virus encontrados para ambas temporadas fueron: Virus del mosaico del pepino (CMV) 23,3%; Virus del bronceado del tomate (TSWV) 20,8%; Virus del mosaico de la alfalfa (AMV) 14,8%;Virus Y de la papa (PVY) 14,5% e Impatients necrotic spot virus (INSV) 3,1% (primera identificación de este virus en pimiento en Chile); Virus del mosaico del tomate (ToMV) 2,2% y Virus del mosaico del tabaco (TMV) 4,9%. Los resultados indicaron que al menos el 30% de las muestras estaba infectada por dos o más virus. La incidencia de virus fue variable en las diferentes épocas de muestreo y localidades y fue severamente afectada por la condición de las plantas al momento del trasplante. Cuando un cultivo se inició con plantas sanas la infección por virus fue menor al finalizar la temporada. El trips de california, Frankliniella occidentalis (Pergande), fue consi