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Detection of Plasmodium in faeces of the New World primate Alouatta clamitans
Plasmodium falciparum and Plasmodium vivax have evolved with host switches between non-human primates (NHPs) and humans. Studies on the infection dynamics of Plasmodium species in NHPs will improve our understanding of the evolution of these parasites; however, such studies are hampered by the diffi...
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Published in: | Memórias do Instituto Oswaldo Cruz 2018-02, Vol.111 (9) |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Plasmodium falciparum and Plasmodium vivax have evolved with host
switches between non-human primates (NHPs) and humans. Studies on the
infection dynamics of Plasmodium species in NHPs will improve our
understanding of the evolution of these parasites; however, such
studies are hampered by the difficulty of handling animals in the
field. The aim of this study was to detect genomic DNA of Plasmodium
species from the faeces of New World monkeys. Faecal samples from 23
Alouatta clamitans from the Centre for Biological Research of Indaial
(Santa Catarina, Brazil) were collected. Extracted DNA from faecal
samples was used for molecular diagnosis of malaria by nested
polymerase chain reaction. One natural infection with Plasmodium simium
was identified by amplification of DNA extracted from the faeces of A.
clamitans. Extracted DNA from a captive NHP was also used for parasite
genotyping. The detection limit of the technique was evaluated in vitro
using an artificial mixture of cultured P. falciparum in NHP faeces and
determined to be 6.5 parasites/µL. Faecal samples of New World
primates can be used to detect malaria infections in field surveys and
also to monitor the genetic variability of parasites and dynamics of
infection. |
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ISSN: | 1678-8060 |