Formulation and characterization of a novel, photoinitiated small intestinal sub-mucosal wound-healing hydrogel

Purpose: To design and characterize a novel 3-D photo-initiated small intestinal sub-mucosal (SIS) hydrogel for use as a scaffold. Methods: Two concentrations of hydrogel were used: 10 mg/mL SIS gel (designated as 1 % hydrogel) and 20 mg/mL SIS gel (designated as 2 % hydrogel). Cross-sections of the...

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Bibliographic Details
Published in:Tropical journal of pharmaceutical research 2022-05, Vol.16 (7)
Main Authors: Liu, Gongbo, Li, Qingtao, Wang, Wenping, Jiang, Junzi, Liao, Jiangui, Ge, Liangpeng, Cao, Chuan, Li, Shirong
Format: Article
Language:English
Subjects:
Extracellular matrix
Hydrogel
Small intestinal sub-mucosa
Wound healing
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Summary:Purpose: To design and characterize a novel 3-D photo-initiated small intestinal sub-mucosal (SIS) hydrogel for use as a scaffold. Methods: Two concentrations of hydrogel were used: 10 mg/mL SIS gel (designated as 1 % hydrogel) and 20 mg/mL SIS gel (designated as 2 % hydrogel). Cross-sections of the hydrogels were examined by scanning electron microscope. In vitro cell culture was carried out on the hydrogels, and cell count was obtained on each hydrogel at different time points. In addition, hematoxylin-eosin (H&E) staining was used to assess in vivo biodegradability of the gels, as well as tissue regeneration. Results: The 1 % hydrogel possessed a larger pore size (143 ± 22 μm) than the 2 % hydrogel (113 ± 17 μm) and showed significantly higher biodegradation rate (22.79 ± 2.47 % of gel left on day 5) than 2% hydrogel (35.37 ± 4.51 % of gel left on day 5) (p < 0.05). However, results from cell culture showed that the 2 % hydrogel had better biocompatibility than 1 % hydrogel. In vivo data revealed that the gels supported cell growth (cell count on days 3 and 5 were 48.33 ± 17.61 and 105.67 ± 21.36, respectively). Conclusion: These results suggest that SIS hydrogels have a high potential for application in tissue regeneration.
ISSN:1596-5996