Antimicrobial Activity of Various Extracts of Ocimum Basilicum L. and Observation of the Inhibition Effect on Bacterial Cells by Use of Scanning Electron Microscopy

The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Ocimum basilicum L. were studied. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. The results indicated that the methanol extracts o...

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Bibliographic Details
Published in:African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2009-01, Vol.5 (4)
Main Authors: Kaya, Ilhan, Yiğit, Nazife, Benli, Mehlika
Format: Article
Language:English
Subjects:
Antimicrobial activity, Disc diffusion method, Ocimum basilicum, Plant extract, SEM, Spices
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Summary:The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Ocimum basilicum L. were studied. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. The results indicated that the methanol extracts of O. basilucum exhibited the antimicrobial activity against tested microorganisms. While the chloroform and acetone extracts had no effect, the methanol extracts showed inhibition zones against strains of Pseudomonas aeruginosa , Shigella sp., Listeria monocytogenes , Staphylococcus aureus and two different strains of Escherichia coli . The cells of microorganisms, which were treated and untreated with plant extracts, were observed by using the scanning electron microscope. It was observed that the treated cells were damaged.
ISSN:0189-6016