Phylogenetic identification and microbial diversity in snow of the summit （8201 m） of Cho Oyu Mountain, Tibet

The bacterial diversity and abundance in snow of the summit （8201 m） of Cho Oyu mountain, Tibet, were analyzed by 16S rRNA gene sequencing followed by scanning electronic microscopy analysis. Most of bacteria were found to be of spherical or oval shape （〉95%）. Bacterial 16S rDNA sequences were class...

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Bibliographic Details
Published in:Chinese science bulletin 2008, Vol.53 (21), p.3317-3323
Main Author: TONG XiaoMei CHEN Fang YU Jun HUA Sang ASAN Ciren LUOSANG JiangBai WANG Wei YU Liang ZHENG XiaoGuang WANG Jian
Format: Article
Language:English
Subjects:
16S
rRNA
基因表达
微生物
雪化干扰
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Summary:The bacterial diversity and abundance in snow of the summit （8201 m） of Cho Oyu mountain, Tibet, were analyzed by 16S rRNA gene sequencing followed by scanning electronic microscopy analysis. Most of bacteria were found to be of spherical or oval shape （〉95%）. Bacterial 16S rDNA sequences were classified into 5 genera （Caulobacter, Ralstonia, Cupriavidus, Pelomonas and Pseudomonas）. Gammaproteobacteria were the most abundant （91.25%） among the library that consists of 594 clones. The sequences found in this study are highly similar to those previously retrieved from other cold environments, such as ice core, sea ice, permafrost and snow. The results showed that the cold and barren environments strongly influence the survival of bacteria. The high similarity among sequences retrieved from snow sample and other places, such as ocean, soil and water, suggested that the bacteria in snow, soil and water environments have the same origin.
ISSN:1001-6538
1861-9541