Effects of tributyltin （TBT） on enzyme activity and oxidative stress in hepatopancreas and hemolymph of small abalone, Haliotis diversicolor supertexta

• Published in: Chinese journal of oceanology and limnology 2009, Vol.27 (4), p.816-824
• Main Author: 贾锡伟 张子平 王淑红 林鹏 邹志华 黄邦钦 王艺磊
• Format: Article
• Language: English
• Subjects: TBT; 三丁基锡; 九孔鲍; 活性水; 肝胰腺; 血淋巴; 超氧化物歧化酶活性
• ISSN: 0254-4059; 1993-5005

We investigated the effect of tributyltin （TBT） exposure on the concentration of malondialdehyde （MDA） and the activity levels of the superoxide dismutase （SOD）, catalase （CAT）, and acid and alkaline phosphatase （ACP and AKP） enzymes in the small abalone, Haliotis diversicolor supertexta. We collected samples of the hepatopancreas and hemolymph 2, 6, 24, 48, 96, and 192 h after exposure to 0.35 μg （Sn）/L TBT. In the hepatopancreas, ACP activity was significantly higher in animals exposed to TBT 2, 24, and 96 h post-exposure compared with the control animals. AKP activity was also higher after 2 h, but SOD and CAT activity was unchanged. The concentration of MDA in the hemolymph was significantly higher than the control animals 2 and 6 h post-exposure. In the hemolymph of animals exposed to TBT, ACP activity was significantly lower than in the control animals 192 h post-exposure, whereas AKP activity was significantly lower 2 and 192 h post-exposure. Hemolymph SOD activity and levels of MDA were significantly lower than in the control animals 24 h after exposure but significantly higher after 96 h. Our results demonstrate that exposure to TBT cause rapid changes in ACP and AKP activity as well as altering the concentration of MDA in the hepatopancreas and hemolymph. SOD and CAT do not appear to be involved in the detoxification of TBT in the hepatopancreas of small abalone.