Expression patterns of Cavl.3 channels in the rat cochlea

• Published in: 生物化学与生物物理学报：英文版 2012, Vol.44 (6), p.513-518
• Main Author: Jin Chen Hanqi Chu Hao Xiong Qingguo Chen Liangqiang Zhou Dan Bing Yun Liu Yan Gao Shaoli Wang Xiaowen Huang Yonghua Cui
• Format: Article
• Language: English
• Subjects: RT-PCR法; Western印迹; 大鼠; 渠道; 神经节细胞; 耳蜗; 螺旋神经节; 表达模式
• ISSN: 1672-9145; 1745-7270

Car1.3 channels are known to be essential for neuronal excitation and signal transduction in the auditory system, their expression patterns in the cochlea are still not fully understood, particularly in the regions where non-sensory cells are located. We performed immu- nohistochemistry, western blotting and reverse transcrip- tion-polymerase chain reaction （RT-PCR） to identify the expression and distribution of Cavl.3 channels in the rat cochlea. Immunohistochemistry revealed that Carl.3 channels were localized in the outer hair cells （OHCs）, inner hair cells （IHCs）, limbus laminae spiralis, spiral ganglion cell, spiral ligament （SL）, and stria vascularis （STV）. The results of RT-PCR and western blotting demonstrated Cavl.3 channels had a tissue-specific ex- pression pattern. Cavl.3 mRNA and protein were inten- sively expressed in the basilar membrane and spiral ganglion while moderate level of Carl.3 channels was observed in SL and STV. Our study preliminarily revealed the expression patterns of Cavl.3 channels in the rat cochlea, providing a theoretical basis for further re- search on the role of Cavl.3 channels in the periphery auditory system.