Synthesis and Applications of Tetra-functional Branched Poly（ethylene glycol） Derivative for the Decellularized Valve Leaflets Cross-linking

• Published in: 武汉理工大学学报：材料科学英文版 2015 (1), p.193-197
• Main Author: HU Xingjian DONG Nianguo SHI Jiawei LI Huadong DENG Cheng LU Cuifen
• Format: Article
• Language: English
• Subjects: 交联剂; 合成; 应用; 支链; 机械性能; 细胞; 聚乙二醇二丙烯酸酯; 衍生物
• ISSN: 1000-2413; 1993-0437

To investigate the effects of polyethylene glycol cross-linking on the mechanical properties, 80 porcine aortic valves were harvested, decellularized, and introduced with sulflaydryl. Then the valves were randomly assigned into 5 experimental groups and 1 control group （n=16）. For the valves in those experimental groups, branched polyethylene glycol diacrylate （PEG） of 5 different molecular weights （3.4, 8, 12, 20, 40 kDa） were synthesized and cross-linked with them respectively. The efficiency of the cross-linking was determined by measuring the amount of residual thiol group and the mechanical properties of the cross-linked valve leaflets were assessed by uni-axial planar tensile testing. The efficiency of the PEG 20 kDa group was 70.72±2.33%, obviously superior to that of the other groups （p〈0.05）. Tensile test proved that branched PEG cross-linking can significantly enhance the mechanical behaviors of the deeellularized valve leaflet and the Young＇s modulus of each group was positively correlated with the molecular weight of PEG. It was concluded that branched PEG with the molecular weight of 20 kDa can effectively cross-link the decellularized porcine aortic valves and improve their mechanical properties, which makes it a promising cross-linker that can be used in the modification of decellularized tissue engineering valves.