Sexual behavior and male volatile compounds in wild and mass-reared strains of the Mexican fruit fly Anastrepha ludens （Diptera： Tephritidae） held under different colony management regimes

• Published in: 中国昆虫科学：英文版 2016 (1), p.105-116
• Main Author: Carlos Felipe Bosa Leopoldo Cruz-Lopez Cristina Silvia Zepeda-Cisneros Javier Valle-Mora Karina Guillen-Navarro Pablo Liedo
• Format: Article
• Language: English
• Subjects: 实蝇; 性行为; 挥发性化合物; 男性; 管理制度; 群体饲养; 规模饲养; 野生菌株
• ISSN: 1672-9609; 1744-7917

We compared the calling and mating behavior and volatile release of wild males Anastrepha ludens （Loew） with males from 4 mass-reared strains： （i） a stan- dard mass-reared colony （control）, （ii） a genetic sexing strain （Tap-7）, （iii） a colony started from males selected on their survival and mating competitiveness abilities （selected）, and （iv） a hybrid colony started by crossing wild males with control females. Selected and wild males were more competitive, achieving more matings under field cage conditions. Mass-reared strains showed higher percentages of pheromone calling males under field conditions except for Tap-7 males, which showed the highest percentages of pheromone calling males under laboratory cage conditions. For mature males of all strains, field-cage calling behavior increased during the last hour before sunset, with almost a 2 fold increase exhibited by wild males during the last half hour. The highest peak mating activity of the 4 mass-reared strains occurred 30 rain earlier than for wild males. By means of solid phase microextraction （SPME） plus gas chromatography-mass spectrometry （GC- MS）, the composition of volatiles released by males was analyzed and quantified, Wild males emitted significantly less amounts of （E,E）-α-farnesene but emitted significantly more amounts of （E,E）-suspensolide as they aged than mass-reared males. Within the 4 mass-reared strains, Tap-7 released significantly more amounts of （E,E）-α-farnesene and hybrid more of （E,E）-suspensolide. Differences in chemical composition could be ex- plained by the intrinsic characteristics of the strains and the colony management regimes. Characterization of calling behavior and age changes of volatile composition between wild and mass-reared strains could explain the differences in mating competitiveness and may be useful for optimizing the sterile insect technique in A. ludens.