QTL mapping revealed TaVp-IA conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

• Published in: 农业科学学报：英文版 2017, Vol.16 (2), p.435-444
• Main Author: ZHOU Sheng-hui FU Lin WU Qiu-hong CHEN Jiao-jiao CHEN Yong-xing XIE Jing-zhong WANG Zhen-zhong WANG Guo-xin ZHANG De-yun LIANG Yong ZHANG Yan YOU Ming-shan LIANG Rong-qi HAN Jun LIU Zhi-yong
• Format: Article
• Language: English
• Subjects: QTL作图; 分子标记辅助选择; 单核苷酸多态性; 发芽小麦; 基因组分析; 复合区间作图法; 显示; 群体
• ISSN: 2095-3119; 2352-3425

Pre-harvest sprouting （PHS） occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci （QTL） for PHS resistance were mapped using an available high-density single nucleotide polymorphism （SNP） and simple sequence repeat （SSR） genetic linkage map developed from a 269 recombinant inbred lines （RILs） population of Yanda 1817xBeinong 6. Using phenotypic data on two locations （Beijing and Shijiazhuang, China） in two years （2012 and 2013 harvesting seasons）, five QTLs, designated as QPhs.cau-3A. 1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau-4A, and QPhs.cau-6A, for PHS （GP） were detected by inclusive composite interval mapping （ICIM） （LOD≥2.5）. Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29-21.65% and 4.36-5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection （MAS）.