C1 inhibitor deficiency enhances contact pathway–mediated activation of coagulation and venous thrombosis

• Published in: Blood 2023-05, Vol.141 (19), p.2390-2401
• Main Authors: Grover, Steven P., Kawano, Tomohiro, Wan, Jun, Tanratana, Pansakorn, Polai, Zsofia, Shim, Young J., Snir, Omri, Brækkan, Sigrid, Dhrolia, Sophia, Kasthuri, Rohan R., Bendapudi, Pavan K., McCrae, Keith R., Wolberg, Alisa S., Hansen, John-Bjarne, Farkas, Henriette, Mackman, Nigel
• Format: Article
• Language: English
• Subjects: Angioedemas, Hereditary - genetics; Animals; Blood Coagulation; Complement C1 Inhibitor Protein - genetics; Humans; Mice; Thrombin; Thrombosis - etiology; Venous Thrombosis - etiology
• ISSN: 0006-4971; 1528-0020; 1528-0020
• DOI: 10.1182/blood.2022018849

•Contact pathway–initiated thrombin generation is increased in C1INH-deficient humans and mice.•Venous, but not arterial, thrombosis is increased in C1INH-deficient mice. [Display omitted] C1 inhibitor (C1INH) is a multifunctional serine protease inhibitor that functions as a major negative regulator of several biological pathways, including the contact pathway of blood coagulation. In humans, congenital C1INH deficiency results in a rare episodic bradykinin-mediated swelling disorder called hereditary angioedema (HAE). Patients with C1INH deficiency–associated HAE (C1INH-HAE) have increased circulating markers of activation of coagulation. Furthermore, we recently reported that patients with C1INH-HAE had a moderate but significant increased risk of venous thromboembolism. To further investigate the impact of C1INH deficiency on activation of coagulation and thrombosis, we conducted studies using patient samples and mouse models. Plasmas from patients with C1INH-HAE had significantly increased contact pathway–mediated thrombin generation. C1INH-deficient mice, which have been used as a model of C1INH-HAE, had significantly increased baseline circulating levels of prothrombin fragment 1+2 and thrombin-antithrombin complexes. In addition, whole blood from C1INH-deficient mice supported significantly increased contact pathway–mediated thrombin generation. Importantly, C1INH-deficient mice exhibited significantly enhanced venous, but not arterial, thrombus formation. Furthermore, purified human C1INH normalized contact pathway–mediated thrombin generation and venous thrombosis in C1INH-deficient mice. These findings highlight a key role for endogenous C1INH as a negative regulator of contact pathway–mediated coagulation in humans and mice. Further, this work identifies endogenous C1INH as an important negative regulator of venous thrombus formation in mice, complementing the phenotype associated with C1INH-HAE. C1 inhibitor (C1INH) is a serine protease inhibitor that is a negative regulator of several pathways, including the contact pathway of coagulation. Congenital absence of C1INH is the major cause of hereditary angioedema (HAE). Grover et al report that patients with HAE have an increased risk of thrombosis, and in this follow-up study, the authors demonstrate that plasma from patients with HAE leads to increased contact-mediated thrombin generation. Furthermore, C1INH-deficient mice have increased venous thrombosis, which is reversible by C1INH administ