Spatiotemporal distribution of antimicrobial resistant organisms in different water environments in urban and rural settings of Bangladesh

• Published in: The Science of the total environment 2022-07, Vol.831, p.154890-154890, Article 154890
• Main Authors: Asaduzzaman, Muhammad, Rousham, Emily, Unicomb, Leanne, Islam, Md. Rayhanul, Amin, Mohammed Badrul, Rahman, Mahdia, Hossain, Muhammed Iqbal, Mahmud, Zahid Hayat, Szegner, Mark, Wood, Paul, Islam, Mohammad Aminul
• Format: Article
• Language: English
• Subjects: Antimicrobial resistance; Aquatic environment; ESBL E. coli; Geographical information system (GIS); Spatial mapping; Water contamination
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.154890

The spatial distribution of clinically important antibiotic resistant bacteria (ARB) and associated genes is important to identify the environmental distribution of contamination and ‘hotspots’ of antimicrobial resistance (AMR). We conducted an integrated survey of AMR in drinking water, wastewater and surface water (rivers and ponds) in three settings in Bangladesh: rural households, rural poultry farms, and urban food markets. Spatial mapping was conducted via geographic information system (GIS) using ArcGIS software. Samples (n = 397) were analyzed for the presence of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec), carbapenem-resistant E. coli (CR-Ec) and resistance genes (blaCTX-M-1,blaNDM-1). In rural households, 5% of drinking water supply samples tested positive for ESBL-Ec, and a high proportion of wastewater, pond and river water samples were positive for ESBL-Ec (90%, 76%, and 85%, respectively). In poultry farms, 10% of drinking water samples tested positive for ESBL-Ec compared to a high prevalence in wastewater, pond and river water (90%, 68%, and 85%, respectively). CR-Ec prevalence in household wastewater and pond water was relatively low (8% and 5%, respectively) compared to river water (33%). In urban areas, 38% of drinking water samples and 98% of wastewater samples from food markets tested positive for ESBL-Ec while 30% of wastewater samples tested positive for CR-Ec. Wastewaters had the highest concentrations of ESBL-Ec, CR-Ec, blaCTXM-1 and blaNDM-1 and these were significantly higher in urban compared to rural samples (p