Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis

Background The relatively high transfection efficiency of polyethylenimine (PEI) vectors has been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes. According to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of e...

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Bibliographic Details
Published in:The journal of gene medicine 2005-05, Vol.7 (5), p.657-663
Main Authors: Akinc, Akin, Thomas, Mini, Klibanov, Alexander M., Langer, Robert
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Cercopithecus aethiops
Chloroquine - pharmacology
COS Cells
Cytoplasm - metabolism
DNA - administration & dosage
Endosomes - metabolism
gene delivery
Gene therapy
Gene Transfer Techniques
Genetic Vectors - metabolism
Hydrogen-Ion Concentration
Luciferases - metabolism
lysosome
Macrolides - pharmacology
Polyethyleneimine - analogs & derivatives
Polyethyleneimine - pharmacology
polyethylenimine (PEI)
Polylysine - metabolism
Polymers - metabolism
Proton Pump Inhibitors
proton sponge
transfection
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