Exploiting the aglycon promiscuity of glycosyltransferase Bs-YjiC from Bacillus subtilis and its application in synthesis of glycosides

•Glycosyltransferase Bs-YjiC from B. subtilis 168 was expressed in E. coli.•Bs-YjiC showed aglycon promiscuity toward 19 structurally diverse compounds.•Twenty-four glycosylated products of 17 aglycons were purified from scale-up reactions.•Bs-YjiC could catalyse the formation of O-, N-, and S-linka...

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Bibliographic Details
Published in:	Journal of biotechnology 2017-04, Vol.248, p.69-76
Main Authors:	Dai, Longhai, Li, Jiao, Yao, Peiyuan, Zhu, Yueming, Men, Yan, Zeng, Yan, Yang, Jiangang, Sun, Yuanxia
Format:	Article
Language:	English
Subjects:	Aglycon promiscuity Arabidopsis thaliana Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacillus subtilis 168 Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism biocatalysts biosynthesis Escherichia coli - genetics functional diversity glucose glycosides Glycosides - chemistry Glycosides - metabolism Glycosylation Glycosyltransferase sucrose synthase Glycosyltransferases - chemistry Glycosyltransferases - genetics Glycosyltransferases - metabolism Natural products nuclear magnetic resonance spectroscopy pterostilbene Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Stilbenes - chemistry Stilbenes - metabolism sucrose synthase uridine uridine diphosphate
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