N‐ to C‐Glycoside Rearrangement of Uridine 5′‐Phosphate in Two Enzymatic Steps for the Production of Pseudouridine 5′‐Phosphate

Pseudouridine (Ψ) is an essential building block of synthetic RNA for medical applications, so methods for its efficient production receive increased interest. Reverse reaction of the Ψ‐5′‐phosphate (ΨMP) C‐glycosidase, that is, d‐ribose 5‐phosphate (Rib5P) + uracil (Ura) → ΨMP + H2O, allows for the...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2025-09, Vol.122 (9), p.2456-2464
Main Authors: Pfeiffer, Martin, Guld, Franziska, Nidetzky, Bernd
Format: Article
Language:English
Subjects:
cascade biocatalysis
Cascade chemical reactions
Catalysis
E coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - metabolism
Glycosidases
Glycosides
Glycosides - chemistry
Glycosides - metabolism
Nucleotides
Phosphates
Productivity
pseudouridine
Pseudouridine - biosynthesis
Pseudouridine - chemistry
Pseudouridine - metabolism
pseudouridine 5’‐phosphate
purine/pyrimidine nucleotide 5’‐phosphate nucleosidase (EC 3.2.2.10)
Ribose
ribose 5’‐phosphate
Uracil
Uridine
uridine 5’‐phosphate
Uridine monophosphate
Uridine Monophosphate - chemistry
Uridine Monophosphate - metabolism
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