Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T 3 , L‐T 4 , and D‐T 4

• Published in: Chirality (New York, N.Y.) N.Y.), 2015-12, Vol.27 (12), p.973-978
• Main Authors: Mitrofan, Grigorina, Stefan‐van Staden, Raluca‐Ioana, Comnea‐Stancu, Ionela Raluca, van Staden, Jacobus Frederick, Bazylak, Grzegorz, Kapnissi‐Christodoulou, Constantina P., Aboul‐Enein, Hassan Y.
• Format: Article
• Language: English
• ISSN: 0899-0042; 1520-636X
• DOI: 10.1002/chir.22537

A fast screening method of whole blood was proposed for enantiorecognition of free L‐T 3 , L‐T 4 , and D‐T 4 . Stochastic microsensors based on four inulins (IN, IQ, TEX, and HD) immobilized on diamond paste (DP) were used for recognition of free L‐T 3 , L‐T 4 , and D‐T 4 . For the enantiorecognition of free L‐T 4 and D‐T 4 in whole blood and pharmaceutical samples, the best microsensor was the one based on TEX/DP (wide linear concentration ranges, and low limits of quantification). The best limit of detection for the assay of free L‐T 3 (400 fmol/L) was recorded using the microsensors based on HD/DP, while for the assay of free L‐T4, and D‐T4 the best limit of determination (1 pmol/L) was recorded using the TX/DP‐based microsensor. For the enantiorecognition of free L‐T 3 in whole blood and pharmaceutical samples the best microsensor was the one based on HD/DP (the wider linear concentration range, and the lower limit of quantification – of pmol/L magnitude order). For the enantiorecognition of free L‐T 3 in whole blood and pharmaceutical samples the best microsensor was the one based on HD/DP (the wider linear concentration range, and the lower limit of quantification – of pmol/L magnitude order). Free L‐T 3 , L‐T 4 , and D‐T 4 were recovered with high reliabilities in whole blood samples (recoveries higher than 99.00%, with RSD values lower than 1.00%) and pharmaceutical samples (recoveries higher than 95.00% with RSD values lower than 1.00%). Chirality 27:973–978, 2015 . © 2015 Wiley Periodicals, Inc.