Potential of Oil Palm Trunk Sap as a Novel Inexpensive Renewable Carbon Feedstock for Polyhydroxyalkanoate Biosynthesis and as a Bacterial Growth Medium

Utilization of cheap renewable carbon feedstock for polyhydroxyalkanoate (PHA) production not only brings down its production cost but also ensures sustainability. The scope of this study was to evaluate the potential of sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable...

Full description

Saved in:
Bibliographic Details
Published in:Clean : soil, air, water air, water, 2012-03, Vol.40 (3), p.310-317
Main Authors: Lokesh, Bhadravathi Eswara, Hamid, Zubaidah Aimi Abdul, Arai, Takamitsu, Kosugi, Akihiko, Murata, Yoshinori, Hashim, Rokiah, Sulaiman, Othman, Mori, Yutaka, Sudesh, Kumar
Format: Article
Language:English
Subjects:
Bacillus megaterium MC1
Biomass
Carbon feedstock
Earth sciences
Earth, ocean, space
Exact sciences and technology
Hydrology
Hydrology. Hydrogeology
Oil palm industry waste
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Utilization of cheap renewable carbon feedstock for polyhydroxyalkanoate (PHA) production not only brings down its production cost but also ensures sustainability. The scope of this study was to evaluate the potential of sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source for PHA production. OPT sap was found to be nutritionally rich and contained various fermentable sugars (5.5% w/v) as its major constituent. Termite gut isolate, Bacillus megaterium MC1 grew profoundly in mineral medium with OPT sap as carbon source and a cell density of 10.9 g/L was attained after 16 h of cultivation in shake flask cultures. A maximum poly‐3‐hydroxybutyrate [P(3HB)] content (% cell dry weight; CDW) of 30 wt% and a P(3HB) concentration of 3.28 g/L was recorded. Additionally, OPT sap extracted from younger tree trunks with prolonged storage had higher sugar content (10.8% w/v) and, when used as a growth medium without the addition of any nutrients, supported bacterial growth comparable to commercially available media. Oil palm tree trunks sap represents almost all the macro‐ and micronutrients required for good bacterial growth. The results for Bacillus megaterium demonstrate the potential of OPT sap as a promising renewable carbon feedstock for bioplastic production and also as a bacterial growth medium.
ISSN:1863-0650
1863-0669
DOI:10.1002/clen.201000598