Triacylglycerol profiling by using chromatographic techniques

Triacylglycerols (TGs) make up the major part of naturally occurring fats and oils. The composition and fine structure of TGs determine to a large extent the functionality of fats and oils as food ingredients and the physiological effects of fats and oils as component of the human diet. Analysis of...

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Bibliographic Details
Published in:European journal of lipid science and technology 2004-09, Vol.106 (9), p.621-648
Main Authors: Buchgraber, Manuela, Ulberth, Franz, Emons, Hendrik, Anklam, Elke
Format: Article
Language:English
Subjects:
analysis
Biological and medical sciences
Fat industries
Food industries
Fundamental and applied biological sciences. Psychology
GLC
HPLC
SFC
silver-ion chromatography
stereospecific analysis
TLC
Triacylglycerols
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Summary:Triacylglycerols (TGs) make up the major part of naturally occurring fats and oils. The composition and fine structure of TGs determine to a large extent the functionality of fats and oils as food ingredients and the physiological effects of fats and oils as component of the human diet. Analysis of intact TGs is usually performed by chromatographic methods. In this article the application of gas‐liquid chromatography, high‐performance chromatography in normal and reversed phase mode, thin‐layer chromatography and supercritical fluid chromatography for the qualitative and quantitative determination of TGs is reviewed. Emphasis is put on those factors that are decisive for obtaining reliable quantitative data. Furthermore, techniques for the stereospecific analysis of the fatty acid distribution along the glycerol backbone of TGs are presented briefly.
ISSN:1438-7697
1438-9312
DOI:10.1002/ejlt.200400986