Development of a novel two‐dimensional gel electrophoresis protocol with agarose native gel electrophoresis

A new protocol for conducting two‐dimensional (2D) electrophoresis was developed by combining the recently developed agarose native gel electrophoresis with either vertical sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) or flat SDS agarose gel electrophoresis. Our innovative...

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Bibliographic Details
Published in:Electrophoresis 2023-09, Vol.44 (17-18), p.1446-1460
Main Authors: Nakagawa, Masataka, Tomioka, Yui, Sakuma, Chiaki, Kurosawa, Yasunori, Shibata, Takashi, Arakawa, Tsutomu, Akuta, Teruo
Format: Article
Language:English
Subjects:
agar gel electrophoresis
agarose
Antigens
Conalbumin
Cost analysis
dyes
Electrophoresis
Gels
Lysozyme
Mass spectrometry
Monoclonal antibodies
Polyacrylamide
polyacrylamide gel electrophoresis
Proteins
Sodium dodecyl sulfate
two-dimensional gel electrophoresis
Western blotting
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