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Purification of glutathione S‐transferase enzyme from quail liver tissue and inhibition effects of (3a R ,4 S ,7 R ,7a S )‐2‐(4‐(( E )‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1 H ‐4,7‐methanoisoindole‐1,3(2 H )‐dione derivatives on the enzyme activity

The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S‐transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue...

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Published in:Journal of biochemical and molecular toxicology 2018-03, Vol.32 (3)
Main Authors: Temel, Yusuf, Koçyigit, Umit M., Taysı, M. Şerif, Gökalp, Faik, Gürdere, Meliha Burcu, Budak, Yakup, Ceylan, Mustafa, Gülçin, İlhami, Çiftci, Mehmet
Format: Article
Language:English
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Summary:The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S‐transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88‐fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS‐PAGE method and showed a single band. In addition, inhibition effects of (3a R ,4 S ,7 R ,7a S )‐2‐(4‐(( E )‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1 H ‐4,7methanoisoindole‐1,3(2 H )‐dion derivatives ( 1a–g ) were investigated on the enzyme activity. The inhibition parameters (IC 50 and K i values) were calculated for these compounds. IC 50 values of these derivatives ( 1a–e ) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. K i values of these derivatives ( 1a–e ) were calculated in the range of 3.04 ± 0.50 to 131.50 ± 32.50 μM. However, for f and g compounds, the inhibition effects on the enzyme were not found.
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.22034