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Simple single-step single-enzyme synthesis of [14C]-GSH
The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant...
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| Published in: | Journal of labelled compounds & radiopharmaceuticals 2010-03, Vol.53 (3), p.110-112 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 112 |
| container_issue | 3 |
| container_start_page | 110 |
| container_title | Journal of labelled compounds & radiopharmaceuticals |
| container_volume | 53 |
| creator | de Keczer, Steve A. Voronin, Tatyana Yao, Jennifer Zhang, Fang-Jie Masjedizadeh, Mohammad R. |
| description | The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [14C]‐GSH to the disulfide [14C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
An efficient one‐step method for the cell‐free preparation of [14C]‐GSH from readily available reagents: [14C]‐gly and γ‐glu‐cys and catalyzed by glutathione synthetase in a simplified cocktail is described. Copyright © 2010 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jlcr.1735 |
| format | article |
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An efficient one‐step method for the cell‐free preparation of [14C]‐GSH from readily available reagents: [14C]‐gly and γ‐glu‐cys and catalyzed by glutathione synthetase in a simplified cocktail is described. Copyright © 2010 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0362-4803</identifier><identifier>EISSN: 1099-1344</identifier><identifier>DOI: 10.1002/jlcr.1735</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>[14C]-glutathione ; [14C]-glycine ; [14C]-GSH ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Contrast media. Radiopharmaceuticals ; Fundamental and applied biological sciences. Psychology ; GSH synthetase ; L-γ-glutamyl-L-cysteine ; Medical sciences ; Methods. Procedures. Technologies ; Pharmacology. Drug treatments</subject><ispartof>Journal of labelled compounds & radiopharmaceuticals, 2010-03, Vol.53 (3), p.110-112</ispartof><rights>Copyright © 2010 John Wiley & Sons, Ltd.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2975-e9a0824622a1da4c15305692abf770ae7f25cb28438bc2ea87bb30c883441f9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22757455$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>de Keczer, Steve A.</creatorcontrib><creatorcontrib>Voronin, Tatyana</creatorcontrib><creatorcontrib>Yao, Jennifer</creatorcontrib><creatorcontrib>Zhang, Fang-Jie</creatorcontrib><creatorcontrib>Masjedizadeh, Mohammad R.</creatorcontrib><title>Simple single-step single-enzyme synthesis of [14C]-GSH</title><title>Journal of labelled compounds & radiopharmaceuticals</title><addtitle>J Label Compd Radiopharm</addtitle><description>The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [14C]‐GSH to the disulfide [14C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
An efficient one‐step method for the cell‐free preparation of [14C]‐GSH from readily available reagents: [14C]‐gly and γ‐glu‐cys and catalyzed by glutathione synthetase in a simplified cocktail is described. Copyright © 2010 John Wiley & Sons, Ltd.</description><subject>[14C]-glutathione</subject><subject>[14C]-glycine</subject><subject>[14C]-GSH</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Contrast media. Radiopharmaceuticals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GSH synthetase</subject><subject>L-γ-glutamyl-L-cysteine</subject><subject>Medical sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>Pharmacology. Drug treatments</subject><issn>0362-4803</issn><issn>1099-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp1kF9LwzAUxYMoOKcPfoO9-OBDtvxt0kcp2ilDYVMGioQ0JprZdSUZaP30tnTuzad74fzO4Z4LwDlGY4wQmaxKE8ZYUH4ABhilKcSUsUMwQDQhkElEj8FJjCuEWo2xARALv65LO4q-ei8tjFtb_-22-mnWrdJU2w8bfRxt3OgFs-wV5ovpKThyuoz2bDeH4Onm-jGbwtlDfptdzaAhqeDQphpJwhJCNH7TzGBOEU9SogsnBNJWOMJNQSSjsjDEaimKgiIjZXs1dmlBh-CyzzVhE2OwTtXBr3VoFEaqa6y6xqpr3LIXPVvraHTpgq6Mj3sDIYILxjtu0nNfvrTN_4HqbpbNd8mwd_j2Qd97hw6fKhFUcLW8z1XyPEfzJV-olP4CnD5y2A</recordid><startdate>201003</startdate><enddate>201003</enddate><creator>de Keczer, Steve A.</creator><creator>Voronin, Tatyana</creator><creator>Yao, Jennifer</creator><creator>Zhang, Fang-Jie</creator><creator>Masjedizadeh, Mohammad R.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201003</creationdate><title>Simple single-step single-enzyme synthesis of [14C]-GSH</title><author>de Keczer, Steve A. ; Voronin, Tatyana ; Yao, Jennifer ; Zhang, Fang-Jie ; Masjedizadeh, Mohammad R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2975-e9a0824622a1da4c15305692abf770ae7f25cb28438bc2ea87bb30c883441f9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>[14C]-glutathione</topic><topic>[14C]-glycine</topic><topic>[14C]-GSH</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Contrast media. Radiopharmaceuticals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GSH synthetase</topic><topic>L-γ-glutamyl-L-cysteine</topic><topic>Medical sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>Pharmacology. Drug treatments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Keczer, Steve A.</creatorcontrib><creatorcontrib>Voronin, Tatyana</creatorcontrib><creatorcontrib>Yao, Jennifer</creatorcontrib><creatorcontrib>Zhang, Fang-Jie</creatorcontrib><creatorcontrib>Masjedizadeh, Mohammad R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of labelled compounds & radiopharmaceuticals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Keczer, Steve A.</au><au>Voronin, Tatyana</au><au>Yao, Jennifer</au><au>Zhang, Fang-Jie</au><au>Masjedizadeh, Mohammad R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple single-step single-enzyme synthesis of [14C]-GSH</atitle><jtitle>Journal of labelled compounds & radiopharmaceuticals</jtitle><addtitle>J Label Compd Radiopharm</addtitle><date>2010-03</date><risdate>2010</risdate><volume>53</volume><issue>3</issue><spage>110</spage><epage>112</epage><pages>110-112</pages><issn>0362-4803</issn><eissn>1099-1344</eissn><abstract>The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [14C]‐GSH to the disulfide [14C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
An efficient one‐step method for the cell‐free preparation of [14C]‐GSH from readily available reagents: [14C]‐gly and γ‐glu‐cys and catalyzed by glutathione synthetase in a simplified cocktail is described. Copyright © 2010 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jlcr.1735</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0362-4803 |
| ispartof | Journal of labelled compounds & radiopharmaceuticals, 2010-03, Vol.53 (3), p.110-112 |
| issn | 0362-4803 1099-1344 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | [14C]-glutathione [14C]-glycine [14C]-GSH Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Contrast media. Radiopharmaceuticals Fundamental and applied biological sciences. Psychology GSH synthetase L-γ-glutamyl-L-cysteine Medical sciences Methods. Procedures. Technologies Pharmacology. Drug treatments |
| title | Simple single-step single-enzyme synthesis of [14C]-GSH |
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