Thermodynamic studies on DNA-dye complexes

The interaction between DNA and dyes such as acridine orange (1), proflavine (2), and 3,8‐diamino‐5‐ethyl‐6‐phenylphenanthridinium bromide (3) were studied by means of the heat of mixing and UV spectra. From the results obtained, the thermodynamic quantities of DNA‐dye complexes were estimated. It i...

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Bibliographic Details
Published in:Die Makromolekulare Chemie 1980-10, Vol.181 (10), p.2175-2181
Main Authors: Tanaka, Shigeyuki, Baba, Yoshihiro, Kagemoto, Akihiro, Fujishiro, Ryoichi
Format: Article
Language:English
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Summary:The interaction between DNA and dyes such as acridine orange (1), proflavine (2), and 3,8‐diamino‐5‐ethyl‐6‐phenylphenanthridinium bromide (3) were studied by means of the heat of mixing and UV spectra. From the results obtained, the thermodynamic quantities of DNA‐dye complexes were estimated. It is concluded that the intercalation process forms a more stable binding than the stacking process does. Further, in each process, ΔG of DNA‐2 and DNA‐3 systems is nearly equal to that of the DNA‐1 system, but their — ΔH and — ΔS values are higher than those of the DNA‐1 system. The stability of binding in DNA‐2 and DNA‐3 systems may be governed largely by ΔH, while in the DNA‐1 system ΔS may contribute largely to the stability of binding.
ISSN:0025-116X
0025-116X
DOI:10.1002/macp.1980.021811014