Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation

Escherichia coli BL21 (DE3) and W3110 strains, belonging to the family B and K-12, respectively, have been most widely employed for recombinant protein production. During the excretory production of recombinant proteins by high cell density cultivation (HCDC) of these strains, other native E. coli p...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2008-05, Vol.8 (10), p.2089-2103
Main Authors: Xia, Xiao-Xia, Han, Mee-Jung, Lee, Sang Yup, Yoo, Jong-Shin
Format: Article
Language:English
Subjects:
Bacterial Outer Membrane Proteins - analysis
Chromatography, Liquid
Electrophoresis, Gel, Two-Dimensional
Escherichia coli
Escherichia coli - cytology
Escherichia coli - metabolism
Escherichia coli Proteins - analysis
Extracellular proteome
High cell density cultivation
Porins - analysis
Proteome - analysis
Proteomics - methods
Species Specificity
Tandem Mass Spectrometry
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Summary:Escherichia coli BL21 (DE3) and W3110 strains, belonging to the family B and K-12, respectively, have been most widely employed for recombinant protein production. During the excretory production of recombinant proteins by high cell density cultivation (HCDC) of these strains, other native E. coli proteins were also released. Thus, we analyzed the extracellular proteomes of E. coli BL21 (DE3) and W3110 during HCDC. E. coli BL21 (DE3) released more than twice the amount of protein compared with W3110 during HCDC. A total of 204 protein spots including 83 nonredundant proteins were unambiguously identified by 2-DE and MS. Of these, 32 proteins were conserved in the two strains, while 20 and 33 strain-specific proteins were identified for E. coli BL21 (DE3) and W3110, respectively. More than 70% of identified proteins were found to be of periplasmic origin. The outer membrane proteins, OmpA and OmpF, were most abundant. Two strains showed much different patterns in their released proteins. Also, cell density-dependent variations in the released proteins were observed in both strains. These findings summarized as reference proteome maps will be useful for studying protein release in further detail, and provide new strategies for enhanced excretory production of recombinant proteins.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200700826