Molecular identification of aStenotrophomonasspecies used in the bioassay for erythromycin in aquaculture samples

• Published in: Molecular and cellular probes 1998-08, Vol.12 (4), p.249-254
• Main Authors: Wang, R.-F, Pothuluri, J.V, Steele, R.S, Paine, D.D, Assaf, N.A, Cerniglia, C.E
• Format: Article
• Language: English
• Subjects: erythromycin detection,Xanthomonas,Stenotrophomonas, 16S rRNA gene sequencing
• ISSN: 0890-8508; 1096-1194
• DOI: 10.1006/mcpr.1998.0178

A bacterial strain isolated from aquaculture pond slurry, which was extremely sensitive to erythromycin, was used to detect erythromycin at levels as low as 0·05 μg ml−1in aquaculture water, sediments and soil samples. Identification of the indicator organism was attempted by 16S rRNA sequencing, biochemical profile, fatty-acid analysis and polymerase chain reaction (PCR). GenBank comparison showed that the 16S rRNA sequence of the strain was similar to those of more than 20 species ofXanthomonasandStenotrophomonas.The position of the strain in a phylogenetic tree based on the 16S rRNA gene sequence comparison is in a cluster ofStenotrophomonas.The fatty-acid analysis also showed that the strain is similar toStenotrophomonas maltophilia.However, the biochemical profile of the strain is most similar toXanthomonas campestris, except that it can utilize maltose, which is similar toS. maltophilia.Polymerase chain reaction results showed that the strain is different fromX. campestris,S. maltophiliaand otherXanthomonasspecies tested. Based on these results, the authors named this strain asStenotrophomonassp. strain NCTR.