The role of the triose-phosphate shuttle and glycolytic intermediates in fatty-acid and glycerolipid biosynthesis in pea root plastids

• Published in: Planta 1994-01, Vol.194 (2), p.193-199
• Main Authors: Qi, Q. (McGill Univ. Ste Anne de Bellevue, Quebec (Canada). Plant Science Dept.), Kleppinger-Sparace, K.F, Sparace, S.A
• Format: Article
• Language: English
• Subjects: ACIDE GRAS; ACIDOS GRASOS; Agronomy. Soil science and plant productions; AZUCARES FOSFATOS; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Energy metabolism; Enzymes; Erbse; FATTY ACIDS; Fettsaeure; Fundamental and applied biological sciences. Psychology; GLICOLISIS; GLYCOLYSE; GLYCOLYSIS; Lipid; Lipid metabolism; LIPIDE; LIPIDOS; LIPIDS; METABOLISM; Metabolism. Physicochemical requirements; METABOLISME; METABOLISMO; Nonesterified fatty acids; Peas; Phosphates; PISUM SATIVUM; Plant physiology and development; PLASTE; Plastid; PLASTIDIOS; PLASTIDS; RACINE; RAICES; ROOTS; Stoffwechsel; SUCRE PHOSPHATE; SUGAR PHOSPHATES; Synthese; Wurzel; Zuckerphosphat
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00196388

The capacity of the triose-phosphate shuttle and various combinations of glycolytic intermediates to substitute for the ATP requirement for fatty-acid and glycerolipid biosynthesis in pea (Pisum sativum L.) root plastids was assessed. In all cases, ATP gave the greatest rates of fatty-acid and glycerolipid biosynthesis. Rates of up to 66 and 27 nmol·(mg protein)-1·h-1 were observed for the incorporation of acetate and glycerol-3-phosphate into lipids in the presence of ATP. In the absence of exogenously supplied ATP, the triose-phosphate shuttle gave up to 44 and 33% of the ATP-control activity in promoting fatty-acid and glycerolipid biosynthesis from acetate and glycerol-3-phosphate, respectively. The optimum shuttle components were 2 mM dihydroxyacetonephosphate (DHAP), 2 mM oxaloacetic acid and 4 mM inorganic phosphate (referred to as the DHAP shuttle). Glyceraldehyde-3-phosphate, as a shuttle triose, was approximately 82% as effective as DHAP in promoting fatty-acid synthesis while 2-phosphoglycerate, 3-phosphoglycerate, and phosphoenolpyruvate were only 27—37% as effective as DHAP. When glycolytic intermediates were used as energy sources for fatty-acid synthesis, in the absence of both exogenously supplied ATP and the triose-phosphate shuttle, phosphoenolpyruvate, 2-phosphoglycerate, fructose-6-phosphate and glucose-6-phosphate each gave 48%, 17%, 23% and 17%, respectively, of the ATP-control activity. Other triose phosphates tested were much less effective in promoting fatty-acid synthesis. When exogenously supplied ATP was supplemented with the DHAP shuttle or glycolytic intermediates, the complete shuttle increased fatty-acid biosynthesis by 37% while DHAP alone resulted in 24% stimulation. Glucose-6-phosphate, fructose-6-phosphate and glycerol-3-phosphate similarly all improved the rates of fatty-acid synthesis by 20—30%. In contrast, 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate all inhibited fatty-acid synthesis by approximately 10% each. The addition of the DHAP shuttle and glycolytic intermediates with or without exogenously supplied ATP caused an increase in the proportion of radioactive oleate and a decrease in the proportion of radioactive palmitate synthesized. The use of these alternative energy sources resulted in higher amounts of free fatty acids and triacylglycerol, and lower amounts of diacylglycerol and phosphatidic acid. The data presented here indicate that ATP is superior in promoting in-vitro fatty-acid biosyn