Cross contamination associated with the use of multiwell culture plates for cytotoxicity assessment of volatile chemicals

In vitro toxicity testing can involve technical problems due to the evaporation of volatile test chemicals. The cytotoxicity of two volatile chemicals (butanol and ethanol) has been assessed with neutral red assay in conventional microtiter plates. The non volatile DMSO chemical is used as a negativ...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 1991-05, Vol.6 (1), p.79-82
Main Authors: BLEIN, O, RONOT, X, ADOLPHE, M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Butanols - chemistry
Butanols - toxicity
Cell Line
Dimethyl Sulfoxide - toxicity
Ethanol - chemistry
Ethanol - toxicity
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Toxicology - instrumentation
Toxicology - methods
Various methods and equipments
Volatilization
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Summary:In vitro toxicity testing can involve technical problems due to the evaporation of volatile test chemicals. The cytotoxicity of two volatile chemicals (butanol and ethanol) has been assessed with neutral red assay in conventional microtiter plates. The non volatile DMSO chemical is used as a negative control. Under these conditions, an important cross contamination between test concentration groups has been observed. This affects cytotoxicity estimation which is overestimated. This cross contamination is prevented when special plates containing removable bars are used.
ISSN:0920-9069
1573-0778
DOI:10.1007/BF00353705