The use of cultured hepatocytes from goats and cattle to investigate xenobiotic oxidative metabolism

The oxidative metabolism of aldicarb (ALD), a carbamate pesticide, and fenbendazole (FBZ), an anthelmintic, was studied using cultured hepatocytes obtained from 4 goats and a bullock and incubated with ALD (50 mumol/L) and FBZ (10 mumol/L). The parent compounds and the metabolites were measured by H...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary research communications 1996, Vol.20 (5), p.449-460
Main Authors: Montesissa, C, Anfossi, P, Van't Klooster, G, Mengelers, M
Format: Article
Language:English
Subjects:
Aldicarb - metabolism
Animals
Anthelmintics - metabolism
Biotransformation
Cattle
Cells, Cultured
Chromatography, High Pressure Liquid
Fenbendazole - metabolism
Goats
Insecticides - metabolism
Kinetics
Liver - metabolism
Male
Xenobiotics - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The oxidative metabolism of aldicarb (ALD), a carbamate pesticide, and fenbendazole (FBZ), an anthelmintic, was studied using cultured hepatocytes obtained from 4 goats and a bullock and incubated with ALD (50 mumol/L) and FBZ (10 mumol/L). The parent compounds and the metabolites were measured by HPLC. Both compounds are metabolized at the sulphur atom via two sequential oxidations, first to the sulphoxide (aldicarb sulphoxide and oxfendazole, respectively) and then to the sulphone. Oxfendazole and fenbendazole sulphone from FBZ, and aldicarb sulphoxide from ALD were found in both species. Aldicarb sulphone was not produced by the hepatocyte preparations from the bullock. The good correlation obtained comparing the in vitro results of FBZ metabolism with published in vivo data obtained on FBZ kinetics in the same species confirmed the usefulness of in vitro models for predictive analysis of in vivo xenobiotic biotransformations.
ISSN:0165-7380
1573-7446
DOI:10.1007/BF00419182