The relationship between zinc protoporphyrin (ZPP) and "free" erythrocyte protoporphyrin (FEP) in lead-exposed individuals

The relationship between zinc protoporphyrin (ZPP) and total erythrocyte protoporphyrin, measured as "free" erythrocyte protoporphyrin (FEP), was determined in 194 adult subjects with different occupational and non-occupational lead exposures. Furthermore, the ZPP-FEP comparison was consid...

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Bibliographic Details
Published in:International archives of occupational and environmental health 1980, Vol.47 (2), p.165-177
Main Authors: Karacić, V, Prpić-Majić, D, Telisman, S
Format: Article
Language:English
Subjects:
Adult
Bilirubin - blood
Erythrocytes - analysis
Female
Humans
Lead Poisoning - blood
Male
Occupational Diseases - blood
Occupational Diseases - chemically induced
Porphyrins - blood
Protoporphyrins - blood
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Summary:The relationship between zinc protoporphyrin (ZPP) and total erythrocyte protoporphyrin, measured as "free" erythrocyte protoporphyrin (FEP), was determined in 194 adult subjects with different occupational and non-occupational lead exposures. Furthermore, the ZPP-FEP comparison was considered with respect to the dose-effect relationship of ZPP and FEP with blood lead (PbB) for males and females, respectively. Bilirubin (Bil.) interferences in ZPP analysis were taken into account. A very close and highly significant relationship (r = 0.962, P < 0.001) was established between ZPP and FEP values. A significant correlation (P < 0.001) between log ZPP or log FEP and PbB (males r = 0.767 and 0.718; females r = 0.525 and 0.405) was also found. It was established, by both in vitro and in vivo studies, that Bil. interferes with the ZPP fluorescence readings; the relationship between "false" positive ZPP concentrations and Bil. concentrations (in vitro r = 0.987, in vivo r = 0.903) was highly significant (P < 0.001). A small but highly significant (r = 0.948, P < 0.001) influence of increased carboxyhemoglobin (COHb) concentrations on the decrease in hematofluorometer ZPP readings, due to inadequate oxygenation of the blood, was found. The results obtained confirm the usefullness of ZPP determinations using hematofluorometers for surveillance of increased lead absorption but stress that the interfering effect of Bil., and to a lesser extent of COHb, cannot be ignored.
ISSN:0340-0131
1432-1246
DOI:10.1007/BF00716375