Studies on dengue 2 virus infection in cyclophosphamide-treated rhesus monkeys

Dengue 2 virus (D2V) replication has been demonstrated in cultured primate mononuclear phagocytes, mitogen treated lymphocytes and lymphoblastoid cells. To determine which of these cell types might play an important role in sustaining infection in vivo, nine rhesus monkeys were immunosuppressed with...

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Bibliographic Details
Published in:Medical microbiology and immunology 1980-02, Vol.168 (1), p.35-47
Main Authors: Marchette, N J, O'Rourke, T, Halstead, S B
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - biosynthesis
Cyclophosphamide - administration & dosage
Cyclophosphamide - pharmacology
Dengue - immunology
Dengue - microbiology
Dengue Virus - growth & development
Disease Models, Animal
Haplorhini
Immunosuppression
Macaca mulatta
Neutrophils - microbiology
Viremia - diagnosis
Virus Replication - drug effects
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Summary:Dengue 2 virus (D2V) replication has been demonstrated in cultured primate mononuclear phagocytes, mitogen treated lymphocytes and lymphoblastoid cells. To determine which of these cell types might play an important role in sustaining infection in vivo, nine rhesus monkeys were immunosuppressed with cyclophosphamide and then infected with D2V. Maintenance dose which held total white blood cell counts to less than 3000/mm3 ablated both primary and secondary antibody responses. Six successfully immunosuppressed animals circulated virus and infected monocytes in blood for prolonged periods. Virus was recovered from lymphatic organs and visualized in tissue mononuclear leukocytes in two subjects dying during the experimental period. The results argue against the hypothesis that lymphoblasts play an important role in dengue virus infection but are consistent with the possibility that mononuclear phagocytes are the site of viral replication in vivo.
ISSN:0300-8584
1432-1831
DOI:10.1007/BF02121650