Rhizobium phaseoli: a molecular genetics view

• Published in: Plant and soil 1988-05, Vol.108 (1), p.179-184
• Main Authors: Martinez, E. (Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos (Mexico). Centro de Investigacion sobre Fijacion de Nitrogeno), Flores, M, Brom, S, Romero, D, Davila, G, Palacios, R
• Format: Article
• Language: English
• Subjects: AGROBACTERIUM; Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; ESTABILIDAD GENETICA; Fundamental and applied biological sciences. Psychology; Genes; GENETIC STABILITY; GENETICA; GENETICS; GENETIQUE; Genomes; Genomics; Molecular genetics; Nitrogen fixation; Nucleotide sequences; PHASEOLUS VULGARIS; Plasmids; RHIZOBIUM; Rhizobium phaseoli; SIMBIOSIS; STABILITE GENETIQUE; SYMBIOSE; SYMBIOSIS; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/BF02370113

We have used molecular genetics techniques to analyze the structural and functional organization of genetic information of Rhizobium phaseoli, the symbiont of the common bean plant Phaseolus vulgaris. As in other Rhizobium species, the genome consists of the chromosome and plasmids of high molecular weight. Symbiotic determinants, nitrogen fixation genes as well as nodulation genes, are localized on a single replicon, the symbiotic (sym) plasmid. The sym plasmid of different R. phaseoli strains was transferred to an Agrobacterium tumefaciens strain cured of its native plasmids. In all cases, Agrobacterium transconjugants able to nodulate bean plants were obtained. Some of the transconjugants had the capacity to elicit an effective symbiosis. The genome of R. phaseoli is complex, containing a large amount of reiterated DNA sequences. In most R. phaseoli strains one of such reiterated DNA families corresponds to the nitrogenase structural genes (nif genes). A functional analysis of these genes suggested that the presence of reiterated nif genes is related to the capacity of fixing atmospheric nitrogen in the symbiotic state. The presence of several repeated sequences in the genome might provide sites for recombination, resulting in genomic rearrangements. By analyzing direct descendants of a single cell in the laboratory, evidence of frequent genomic rearrangements in R. phaseoli was found. We propose that genomic rearrangements constitute the molecular basis of the frequent variability and loss of symbiotic properties in different Rhizobium strains.