Enantiomeric resolution of melatonin ligands receptors by liquid chromatography on amylose chiral stationary phases

Analytical HPLC methods using derivatized amylose chiral stationary phases were developed for the resolution of enantiomers of methoxy an ethyl tetrahydronaphthalenic derivatives, new agonist and antagonist ligands for melatonin receptors. Separation was by normal phase methodology with a mobile pha...

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Bibliographic Details
Published in:Chromatographia 1998-12, Vol.48 (11-12), p.790-796
Main Authors: Vaccher, C, Fourmaintraux, E, Belloli, E, Vaccher, M. p, Bonte, J. P
Format: Article
Language:English
Subjects:
1-propanol
agonists
amylose
Analysis
antagonists
Biological and medical sciences
enantiomers
ethanol
General pharmacology
high performance liquid chromatography
isopropyl alcohol
ligands
Medical sciences
melatonin
methanol
Pharmacology. Drug treatments
receptors
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Summary:Analytical HPLC methods using derivatized amylose chiral stationary phases were developed for the resolution of enantiomers of methoxy an ethyl tetrahydronaphthalenic derivatives, new agonist and antagonist ligands for melatonin receptors. Separation was by normal phase methodology with a mobile phase ofn-hexane-alcohol (methanol, ethanol, 1-propanol or 2-propanol) in various proportions, and a silica-based, amylose tris-(S)-1-phenylethylcarbamate (Chiralpak AS), or tris-3,5-dimethylphenylcarbamate (Chiralpak AD). The mobile phase and the chiral stationary phase were optimized for best resolution. The effects of concentration of alcohol, various aliphatic alcohols in the mobile phase were studied. The effects of substitution were analysed. Baseline separation (Râ‚›>1.5) was easily obtained in man cases.
ISSN:0009-5893
1612-1112
DOI:10.1007/BF02467649