Determination of cisapride in human plasma by high-performance liquid chromatography with fluorescence detection

A new sensitive HPLC-FLD method has been developed and validated for the determination of cisapride in human plasma for a bioequivalence study. A gradient method was used to remove late-eluting plasma components of no interest. The separation was performed on a Li-ChroCART 250-4 Purospher RP-18 (5 μ...

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Bibliographic Details
Published in:Chromatographia 2003, Vol.57 (1-2), p.47-50
Main Authors: Kiss, B. Dalmadi, Nemes, K. Balogh, Klebovich, I
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
calibration
fluorescence
General pharmacology
high performance liquid chromatography
humans
Medical sciences
pharmacokinetics
Pharmacology. Drug treatments
wavelengths
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Summary:A new sensitive HPLC-FLD method has been developed and validated for the determination of cisapride in human plasma for a bioequivalence study. A gradient method was used to remove late-eluting plasma components of no interest. The separation was performed on a Li-ChroCART 250-4 Purospher RP-18 (5 μm particle) analytical column fitted with a LiChroCART 4-4 Purospher RP-18 endcapped (5 μm particle) guard column. The excitation and emission wavelengths were 295 and 350 nm during fluorescence detection. The calibration plot was linear in the range of 5–200 ng mL⁻¹. A demethoxy analogue of cisapride was used as internal standard.
ISSN:0009-5893
1612-1112
DOI:10.1007/BF02497476