A Simple Method for the Recovery of Multiple Fertile Plants from Individual Somatic Embryos of Soybean [Glycine max (L.) Merrill]

We describe a method for obtaining and proliferating multiple, fertile plants from somatic embryos of several experimental and commercial soybean varieties. Shoot-bud cultures were initiated by placing cotyledonary and torpedo-stage somatic embryos derived from immature seedling cotyledons onto Chen...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1991-07, Vol.27P (3), p.153-157
Main Authors: Wright, M. S., Launis, K. L., Novitzky, R., Duesing, J. H., Harms, C T.
Format: Article
Language:English
Subjects:
Cotyledons
Embryos
Genotypes
Immatures
Plant cells
Plants
Regeneration
Somaclonal variation
Somatic embryos
Soybeans
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Summary:We describe a method for obtaining and proliferating multiple, fertile plants from somatic embryos of several experimental and commercial soybean varieties. Shoot-bud cultures were initiated by placing cotyledonary and torpedo-stage somatic embryos derived from immature seedling cotyledons onto Cheng's basal medium (CBO) containing 0.5 to 2.5 mg/liter 6-benzyladenine (6-BA). Prolific masses of adventitious shoots were produced within 6 to 18 wk. These cultures can be propagated indefinitely with regular subcultures to CBO containing 0.5 mg/liter 6-BA. Individual shoots were separated from the clusters and were rooted on CBO medium without exogenous growth regulators. By this method any number of plants can be produced from individual somatic embryos. The risk of losing valuable genotypes (e.g., derived from in vitro selection or transformation) due to inefficient embryo germination and embryo-to-plant conversion is thus greatly reduced. Plants were established in the greenhouse and progenies were field tested. Progenies from shoot-bud culture-derived plants showed no somaclonal variation for the seven recessive marker traits or quantitative agronomic characters evaluated under field conditions.
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02632200