Major injury induces increased production of interleukin-10 in human granulocyte fractions

Patients with severe trauma or polytrauma frequently acquire alterations in immune functions which are correlated to dysbalanced cytokine synthesis. In these settings the role of polymorphonuclear neutrophil granulocytes (PMN) as cytokine-producing cells is less well characterized. The immunosuppres...

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Bibliographic Details
Published in:Langenbeck's archives of surgery 1998-12, Vol.383 (6), p.460-465
Main Authors: Köller, M, Clasbrummel, B, Kollig, E, Hahn, M P, Muhr, G
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Bacterial Toxins
Burns - immunology
Burns - metabolism
Concanavalin A
Enterotoxins
Enzyme-Linked Immunosorbent Assay
Female
Humans
Interleukin-10 - biosynthesis
Interleukin-6 - biosynthesis
Leukocytes, Mononuclear - metabolism
Lymphocyte Activation
Male
Middle Aged
Multiple Trauma - immunology
Multiple Trauma - metabolism
Neutrophils - metabolism
Staphylococcus aureus
Superantigens
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Summary:Patients with severe trauma or polytrauma frequently acquire alterations in immune functions which are correlated to dysbalanced cytokine synthesis. In these settings the role of polymorphonuclear neutrophil granulocytes (PMN) as cytokine-producing cells is less well characterized. The immunosuppressive role of interleukin (IL)-10 is well known, and increased systemic IL-10 levels are related to the severity of injury and to posttraumatic complications. We determined concentrations of IL-10 in culture supernatants of 30 individual PMN fractions isolated from 18 severely traumatized patients (15 polytraumata, Injury Severity Score: 18-41, 3 severely burned patients) admitted to intensive care units. IL-10 was analyzed by ELISA (R&D Systems, Wiesbaden, Germany). PMN were isolated from EDTA-anticoagulated peripheral blood employing a one-step procedure based on a discontinuous double Ficoll gradient. The cells [1 x 10(6)/ml RPMI 1640 supplemented with 10% fetal calf serum and 25 mM N-(2-hydroxyethyl)-piperazine-N'-(2-ethanesulfonic acid] were stimulated with 0.05% heat-killed Staphylococcus aureus (Pansorbin, Calbiochem-Novabiochem, Bad Soden, Germany) for 24 h using cell culture conditions. Our results show that PMN fractions of traumatized patients produce significantly (P
ISSN:1435-2443
1435-2451
DOI:10.1007/s004230050161