Immobilization of horseradish peroxidase on titanate nanowires for biosensing application

• Published in: Journal of applied electrochemistry 2016-01, Vol.46 (1), p.17-25
• Main Authors: Nicolini, João Victor, Ferraz, Helen Conceição, de Resende, Neuman Solange
• Format: Article
• Language: English
• Subjects: Chemistry; Chemistry and Materials Science; Electrochemistry; Industrial Chemistry/Chemical Engineering; Physical Chemistry; Research Article; Sensors and Electrochemical Methods
• ISSN: 0021-891X; 1572-8838
• DOI: 10.1007/s10800-015-0907-z

Horseradish peroxidase (HRP) was immobilized on titanate nanowires (TNW) to produce a peroxide biosensor. Nanowires were synthesized by a simple and cost-effective alkaline hydrothermal method, and characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectra, and N 2 physisorption. In this study, HRP was covalently immobilized on TNW using glutaraldehyde as crosslinking agent after surface treatment with (3-aminopropyl)trimethoxysilane. Electrochemical evaluation of the biosensor was performed by cyclic voltammetry, exhibiting a low reduction potential for H 2 O 2 , around −0.38 V versus Ag/AgCl at pH 7.0, and a low detection limit of 1.2 µmol L −1 . TNW have demonstrated excellent performance as immobilizing support for the enzyme as the biosensor exhibited a storage stability up to 30 days, retaining 91 % of its activity. All together, these results show that the produced biosensor based on the HRP enzyme, and TNW is efficient for the determination of H 2 O 2 , showing a fast and sensitive response. Graphical Abstract