The analysis of lncRNA HOTAIR rs12826786 C>T polymorphism and gastric cancer susceptibility in a Turkish population: lack of any association in a hospital-based case–control study

• Published in: Irish journal of medical science 2017-11, Vol.186 (4), p.859-865
• Main Authors: Ülger, Y., Dadaş, E., Yalinbaş Kaya, B., Sümbül, A. T., Genç, A., Bayram, S.
• Format: Article
• Language: English
• Subjects: Case-Control Studies; Family Medicine; Female; General Practice; Genetic Predisposition to Disease; Humans; Internal Medicine; Male; Medicine; Medicine & Public Health; Middle Aged; Original Article; Polymorphism, Genetic; Polymorphism, Single Nucleotide; RNA, Long Noncoding - metabolism; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Turkey
• ISSN: 0021-1265; 1863-4362
• DOI: 10.1007/s11845-017-1596-x

Background The HOX transcript antisense intergenic RNA ( HOTAIR ), a well-known long noncoding RNA (lncRNA), has been widely identified to participate in pathogenesis of multiple cancers. An aberrant up-regulation and biological functions have been observed in gastric cancer (GC). A common single nucleotide polymorphism (SNP) (rs12826786 C>T) at the HOTAIR has been reported to influence HOTAIR expression, but its association with GC has yet to be investigated in Turkish population. Aim The aim of the present study was to investigate whether HOTAIR rs12826786 C>T polymorphism could be involved in the risk of GC susceptibility in Turkish population. Methods We genotyped HOTAIR rs12826786 C>T polymorphism in 312 Turkish individuals including 105 GC patients and 207 healthy controls matched on age and gender by a Real-Time Polymerase Chain Reaction (PCR) with the TaqMan assay. Results No statistically significant differences were found in the allele or genotype distributions of the HOTAIR rs12826786 C>T polymorphism among GC and healthy control subjects ( P  > 0.05). Conclusions Our results demonstrate that the HOTAIR rs12826786 C>T polymorphism has not been in any major role in genetic susceptibility to gastric carcinogenesis, at least in the population studied here. Independent studies are needed to validate our findings in a larger series, as well as in patients of different ethnic origins.