UHPLC Method for Quantification of Bioactive Components in Fenugreek Herbal Preparations

Trigonella foenum-graecum L., Fabaceae, also known as fenugreek, is a popular herb commonly used as a spice, flavoring, and medicinal agent. It is enriched with bioactive components namely pinitol, 4-hydroxy isoleucine, and raffinose which are proven to be responsible for fenugreek medicinal and hea...

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Bibliographic Details
Published in:Revista brasileira de farmacognosia 2023-10, Vol.33 (5), p.1031-1040
Main Authors: Agrawal, Sristi, Bisen, Amol Chhatrapati, Biswas, Arpon, Jaiswal, Shubham, Choudhury, Abhijit Deb, Gupta, Sapna, Narender, Tadigoppula, Bhatta, Rabi Sankar
Format: Article
Language:English
Subjects:
Medicine
Original Article
Pharmacy
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Summary:Trigonella foenum-graecum L., Fabaceae, also known as fenugreek, is a popular herb commonly used as a spice, flavoring, and medicinal agent. It is enriched with bioactive components namely pinitol, 4-hydroxy isoleucine, and raffinose which are proven to be responsible for fenugreek medicinal and health benefits. The current study focuses on a chemometric guided method development and validation of three components by ultra-high-performance liquid chromatography coupled to a refractive index according to the International Council of Harmonization guidelines using quality by design driven response surface Box Behnken design. The developed method was validated considering linearity, sensitivity, selectivity, limit of quantification, limit of detection, stability, accuracy, and precision. A universal refractive index detection technique was used for quantifying the bioactive components present in the herbal nutraceutical preparations. The Nucleosil NH 2 bonded analytical column maintained at 35 °C was used for chromatographic separation against optimum mobile phase composition of acetonitrile: triple distilled water in the ratio of 7:3, v/v at a flow rate of 0.4 ml/min. The developed method is sensitive enough to detect (0.015 mg/ml), easy, affordable, reproducible, and repeatable. In conclusion, this newly developed method holds great potential for application in various domains such as pharmaceutical industries, drug discovery development, and the measurement of bioactive component concentrations in commercial herbal products intended for human use. Graphical Abstract
ISSN:1981-528X
1981-528X
DOI:10.1007/s43450-023-00431-5