Laboratory-scale purification of microcystins using flash chromatography and reversed-phase high-performance liquid chromatography

Microcystins were extracted from 7 1 (equivalent to 313 g dry weight) of cyanobacterial scum collected from Rutland Water in Leicestershire, UK in 1989. The resulting aqueous extract was rapidly concentrated on a C 18 flash chromatography cartridge and microcystins were eluted using a step gradient....

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Bibliographic Details
Published in:Journal of Chromatography A 1996-05, Vol.734 (1), p.163-173
Main Authors: Edwards, Christine, Lawton, Linda A., Coyle, Sadie M., Ross, Paul
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Chromatography - methods
Chromatography, High Pressure Liquid - methods
Cyanobacteria - chemistry
Enzyme Inhibitors - isolation & purification
Flash chromatography
Microcystins
Molecular Sequence Data
Peptides
Peptides, Cyclic - chemistry
Peptides, Cyclic - isolation & purification
Phosphoprotein Phosphatases - antagonists & inhibitors
Preparative chromatography
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Summary:Microcystins were extracted from 7 1 (equivalent to 313 g dry weight) of cyanobacterial scum collected from Rutland Water in Leicestershire, UK in 1989. The resulting aqueous extract was rapidly concentrated on a C 18 flash chromatography cartridge and microcystins were eluted using a step gradient. Fractions were collected manually and monitored by UV spectrophotometer and analytical HPLC. Fractions containing microcystins of similar polarity were pooled to give three fractions. Simple isocratic methods for separating each fraction were developed on an analytical column and scaled up to a 15×7.5 cm I.D. column. Closed-loop recycling was used to maximise yield and purity of two hydrophobic microcystins.
ISSN:0021-9673
DOI:10.1016/0021-9673(95)01004-1