Enantiomeric separation of zopiclone, its metabolites and products of degradation on a ß-cyclodextrin bonded phase

In this paper the separation of zopiclone (ZP) enantiomers, its degradation products and chiral metabolites, has been investigated on a ß-cyclodextrin bonded phase. The liquid chromatographic behavior of the enantiomers of zopiclone, zopiclone-N-oxide (OXZP) and N-desmethyl-zopiclone (DMZP) has been...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1996-04, Vol.729 (1), p.19-28
Main Authors: Piperaki, Stavroula, Parissi-Poulou, Maria
Format: Article
Language:English
Subjects:
Cyclodextrins
Enantiomer separation
Mobile-phase composition
Optimization
Pharmaceutical analysis
Zopiclone
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper the separation of zopiclone (ZP) enantiomers, its degradation products and chiral metabolites, has been investigated on a ß-cyclodextrin bonded phase. The liquid chromatographic behavior of the enantiomers of zopiclone, zopiclone-N-oxide (OXZP) and N-desmethyl-zopiclone (DMZP) has been studied with respect to mobile-phase composition, pH, ionic strength and the nature of the organic modifier. Based on the results, conditions were chosen for the separation of the enantiomers of zopiclone, its chiral metabolites and its related degradation products. The separation selectivity of a ß-cyclodextrin (ß-CD) bonded-phase column was examined. Further, based on crystallographic data, a computer model of the ZP molecule has been designed by using the HyperChem program in order to estimate the possibility of the different groups to interact with the ß-CD and to give a plausible explanation of the mechanism of chiral discrimination.
ISSN:0021-9673
DOI:10.1016/0021-9673(95)01182-X