Determination of stoichiometric coefficients and apparent formation constants for α- and β-CD complexes of terpenes using reversed-phase liquid chromatography

The stoichiometric coefficients and apparent formation constants for (±)- α-pinene, (±)- β-pinene, (±)-camphene and (±)-limonene with α- and β-cyclodextrins were determined using high-performance liquid chromatography. These measurements were performed in 55:45 0.1% orthophosphoric acid in water-met...

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Bibliographic Details
Published in:Journal of Chromatography A 1996-06, Vol.736 (1), p.1-9
Main Authors: Moeder, C., O'Brien, T., Thompson, R., Bicker, G.
Format: Article
Language:English
Subjects:
Apparent formation constants
Chiral mobile phase additive
Cyclodextrins
Enantiomer separation
Stoichiometric coefficients
Terpenes
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Summary:The stoichiometric coefficients and apparent formation constants for (±)- α-pinene, (±)- β-pinene, (±)-camphene and (±)-limonene with α- and β-cyclodextrins were determined using high-performance liquid chromatography. These measurements were performed in 55:45 0.1% orthophosphoric acid in water-methanol using a Vydac C 4 column (15.0×0.46 cm I.D., 10 μm, 300A). Using α-cyclodetrin (α-CD), 1:2 guest-CD complexes were found for the three bicyclic compounds, α- and β-pinene and camphene; a 1:1 guest-CD complex was found for limonene. Using β-cyclodextrin (β-CD), 1:1 guest-CD complexes were found for all compounds. Recognition of the terpene enantiomers was only achieved for the bicyclic compounds using α-CD. For α- and β-CD, the apparent formation constant for each terpene-CD complex was calculated.
ISSN:0021-9673
DOI:10.1016/0021-9673(95)01276-1