High-yield production of mono- and di-oleylglycerol by lipase-catalyzed hydrolysis of triolein

Lipases from several origins—in soluble form or immobilized on solid supports of different aquaphilicity—have been used for the production of mono- and di-oleylglycerol by hydrolysis of triolein. The porcine pancreatic lipase adsorbed on Celite was found to be the most effective biocatalyst tested....

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Bibliographic Details
Published in:Enzyme and microbial technology 1996, Vol.18 (1), p.66-71
Main Authors: Plou, Francisco J., Barandiarán, María, Calvo, María V., Ballesteros, Antonio, Pastor, Eitel
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Biotransformations
diglycerides
food additives
Fundamental and applied biological sciences. Psychology
hydrolysis of triglycerides
immobilized lipase
Methods. Procedures. Technologies
monoglycerides
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Summary:Lipases from several origins—in soluble form or immobilized on solid supports of different aquaphilicity—have been used for the production of mono- and di-oleylglycerol by hydrolysis of triolein. The porcine pancreatic lipase adsorbed on Celite was found to be the most effective biocatalyst tested. High activity and excellent selectivity toward the formation of intermediate acylglycerols were achieved (the conversion to diolein plus monoolein was 79% after 5 h of reaction). The time course of triolein hydrolysis with free and immobilized lipases was analyzed using a sequential kinetic model, and the apparent first-order rate constants were calculated. The differences found in the rate constants were related to differences in the regioselectivity of the enzymes and/or the aquaphilicity of the supports.
ISSN:0141-0229
1879-0909
DOI:10.1016/0141-0229(96)00054-3