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Stimulative effects of lead on bone resorption in organ culture
To clarify whether hypercalcemia after injection of Pb to rats is due to biological bone resorption or physicochemical mineral dissolution, the effect of lead (Pb) on release of previously incorporated 45Ca in organ culture was investigated. Pb at 50 μM and above stimulated the release of 45Ca and h...
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Published in: | Toxicology (Amsterdam) 1995-03, Vol.97 (1), p.191-197 |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | To clarify whether hypercalcemia after injection of Pb to rats is due to biological bone resorption or physicochemical mineral dissolution, the effect of lead (Pb) on release of previously incorporated
45Ca in organ culture was investigated. Pb at 50 μM and above stimulated the release of
45Ca and hydroxyproline (Hyp). Pb did not stimulate
45Ca release from the bones inactivated by freezing and thawing. Eel calcitonin (ECT), bafilomycin A
1 and scopadulcic acid B (SDB) inhibited Pb-stimulated
45Ca release. These results indicate that Pb-induced
45Ca release is due to osteoclastic bone resorption. Pb-stimulated bone resorption was inhibited by indomethacin and flurbiprofen. Pb stimulated the release of prostaglandin E
2 (PGE
2) from the bones into the media. There was significantly high correlation between
45Ca and PGE
2 release. Pb-induced bone resorption was inferred to be mediated by PGE
2. From these results, it was suggested that hypercalcemia after Pb injection might be caused by biological bone resorption. |
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ISSN: | 0300-483X 1879-3185 |
DOI: | 10.1016/0300-483X(94)02948-T |