Passage of X-ray-induced immortal, non-transformed phenotype by DNA-mediated transfection

To better understand the molecular basis of X-ray-induced carcinogenesis, the immortalization step of this multistep process was examined. Primary rat embryo cells were X-irradiated in vitro and six clones were isolated. Three of these, one designated X-REF-23, were immortal and non-transformed. Tra...

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Bibliographic Details
Published in:Cancer letters 1995-10, Vol.97 (1), p.39-47
Main Authors: Too, Catherine K.L., Sierra-Rivera, Elaine, Oberley, Larry W., Guernsey, Duane L.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Cell Division
DNA - radiation effects
DNA transfection
Immortalization
Medical sciences
Mice
Oncogenes
Phenotype
Physical agents
Primary rodent cells
Rats
Transfection
Tumors
X-Ray
X-Rays
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Summary:To better understand the molecular basis of X-ray-induced carcinogenesis, the immortalization step of this multistep process was examined. Primary rat embryo cells were X-irradiated in vitro and six clones were isolated. Three of these, one designated X-REF-23, were immortal and non-transformed. Transfection of high molecular weight DNA from rat X-REF-23 cells into primary mouse cells yielded two immortal and non-transformed mouse clones, 1K and 2I. Using a 2.3 kb rat-specific repetitive sequence as probe, 1K and 2I were demonstrated to contain rat DNA. This transfected DNA was not any of the known immortalization-associated proto-oncogenes. DNA from 1K was then transfected into primary mouse cells, with or without co-transfection of pSV2 neo DNA. Six immortal mouse clones were isolated and confirmed to contain rat sequences. In conclusion, the immortal phenotype can be transferred by DNA transfection.
ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(95)03949-W