Temperature change of the lamellar structure of DPPC/disaccharide/water systems with low water content

• Published in: Biochimica et biophysica acta 1998-05, Vol.1371 (2), p.223-231
• Main Authors: Nagase, H, Ueda, H, Nakagaki, M
• Format: Article
• Language: English
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Calorimetry, Differential Scanning; Disaccharide; Disaccharides - chemistry; DPPC; DSC; Fourier-transform infrared (FT-IR); Hot Temperature; Membranes, Artificial; Phase transition temperature; Powders; Water - chemistry; X-Ray Diffraction
• ISSN: 0005-2736; 0006-3002; 1879-2642
• DOI: 10.1016/S0005-2736(98)00016-9

Temperature change in l- α-dipalmitoyl phosphatidylcholine (DPPC)/disaccharide systems with low water content (less than 8 wt.%) was investigated using X-ray diffraction within a range of two transition temperatures. X-ray diffraction above the higher transition temperature showed a broad symmetric peak, indicating the L α phase. Below the higher transition temperature, two overlapping diffraction peaks were observed. After peak separation, temperature change in these systems was analyzed using peak parameters of the two peaks. Peak parameters of the lower angle peak changed continuously up to and above the higher transition temperature, suggesting the systems to be in a liquid crystal phase below the higher transition temperature. Fourier-transform infrared (FT-IR) spectra of the DPPC/trehalose system with 5.5 wt.% water showed the wave number of asymmetric stretching of phosphate groups to change at the lower transition temperature and that of symmetric stretching of CH 2 groups, to change between the lower and higher transition temperatures. Thus, below the lower transition temperature, the system is shown to be in a gel phase. Conformational change in phosphate groups occurred at the lower transition temperature. Within the lower and higher transition temperatures, two phases were found to coexist and transition from the gel phase to L α phase to occur continuously. Above the higher transition temperature, the system is in the L α phase.